Abstract
The effect of adrenalectomy (ADX) or saline ingestion, which is a hypertensive factor, on the expression of calcium-binding protein regucalcin mRNA in the kidney cortex of rats was investigated. The change of regucalcin mRNA levels was analyzed by Northern blotting using rat liver regucalcin complementary DNA (0.9 kb of open-reading frame). Regucalcin mRNA was expressed in the kidney cortex but not the medulla. Rats were adrenalectomized, and 48 h later they were sacrificed. ADX caused a reduction of regucalcin mRNA levels in the kidney cortex, suggesting that adrenal glands participate in the regulation of the mRNA expression. This reduction was not restored by the subcutaneous administration of dexamethasone with an effective dose (1 mg/kg body weight), which can stimulate kidney regucalcin mRNA expression. Regucalcin mRNA levels in the kidney cortex of rats were markedly suppressed by the ingestion of saline for 7 days. The ADX-induced decrease of renal cortex regucalcin mRNA levels was not appreciably restored by saline ingestion. Moreover, regucalcin mRNA levels in the kidney cortex of spontaneous hypertensive rats (SHR) were clearly decreased as compared with that of control (Wistar-Kyoto) rats. Meanwhile, calcium content in the kidney cortex was not significantly decreased by ADX or saline ingestion. The present study suggests that the expression of regucalcin mRNA in the kidney cortex of rats is suppressed by saline administration.
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Williamson JR, Cooper RH, Hoek JB: Role of calcium in the hormonal regulation of liver metabolism. Biochim Biophys Acta 639: 243–295,1981
Kraus-Friedman N: Calcium sequestration in the liver. Cell Calcium 11:625–640,1990
Reihart PH, Taylor WM, Bygrave FL: The role of calcium ions in the mechanisms action of α-adrenergic agonists in rat liver. Biochem J 223:1–13,1984
Cheung WY: Calmodulin plays a pivotal role in cellular regulation. Science 202: 19–27, 1984
Nishizuka Y: Studies and perspectives of protein kinase C. Science 233: 305–312, 1986
Mori S, Yamaguchi M: Hepatic calcium-binding protein regucalcin decreases Ca2+/calmodulin-dependent protein kinase activity in rat liver cytosol. Chem Pharm Bull (Tokyo) 38: 2216–2218, 1990
Yamaguchi M, Mori S: Inhibitory effect of calcium-binding protein regucalcin on protein kinase C activity in rat liver cytosol. Biochem Med Metab Biol 43: 140–146, 1990
Yamaguchi M, Sakurai T: Inhibitory effect of calcium binding protein regucalcin on Ca2+-activated DNA fragmentation in rat liver nuclei. FEBS Lett 279: 281–284, 1991
Yamaguchi M: A novel Ca2+-binding protein regucalcin and calcium inhibition: Regulatory role in liver cell function. In: K. Kohama (ed). Calcium Inhibition. Japan Sci Soc Press, Tokyo and CRC Press, Boca Raton, 1992, pp 19–41
Shimokawa N, Yamaguchi M: Molecular cloning and sequencing of the cDNA coding for a calcium-binding protein regucalcin from rat liver. FEBS Lett 327: 251–255, 1993
Shimokawa N, Matsuda Y, Yamaguchi M: Genomic cloning and chromosomal assignment of rat regucalcin gene. Mol Cell Biochem 151: 157–163,1995
Shimokawa N, Yamaguchi M: Expression of hepatic calcium binding protein regucalcin mRNA is mediated through Ca2+/calmodulin in rat liver. FEBS Lett 316: 79–84, 1993
Isogai M, Yamaguchi M: Calcium administration increases calciumbinding protein regucalcin concentration in the liver of rats. Mol Cell Biochem 143: 53–58, 1995
Shimokawa N, Yamaguchi M: Calcium administration stimulates the expression of calcium-binding protein regucalcin mRNA in rat liver. FEBS Lett 305: 151–154, 1992
Yamaguchi M, Isogai M: Tissue concentration of calcium binding protein regucalcin in rats by enzyme-linked immunoadsorbent assay. Mol Cell Biochem 122: 65–68, 1993
Yamaguchi M, Kurota H: Expression of calcium-binding protein regucalcin mRNA in the kidney cortex of rats: The stimulation by calcium administration. Mol Cell Biochem 146: 71–77, 1995
Kurota H, Yamaguchi M: Steroid hormonal regulation of calcium-binding protein regucalcin mRNA expression in the kidney cortex of rats. Mol Cell Biochem 155: 105–111, 1996
Sambrook J, Maniatis T, Fritsch EF: Northern hybridization. In: Molecular Cloning (2nd Edn), Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, pp 7.39–7.52, 1989
Schmidt U, Schmid J, Schmid H, Dubach UC: Sodium- and potassium-activated ATPase: A possible target of aldosterone. J Clin Invest 55:655–660,1975
Van Os CH: Transcellular calcium transport in intestinal and renal epithelial cells. Biochim Biophys Acta 906: 195–222, 1987
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Shinya, N., Kurota, H. & Yamaguchi, M. Calcium-binding protein regucalcin mRNA expression in the kidney cortex is suppressed by saline ingestion in rats. Mol Cell Biochem 162, 139–144 (1996). https://doi.org/10.1007/BF00227541
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DOI: https://doi.org/10.1007/BF00227541