Summary
The origin and phylogeny of the Guinea yams, consisting of the white yam (Dioscorea rotundata Poir.) and the yellow yam (D. cayenensis Lamk.), has been investigated. Fourteen cultivars of Guinea yams were sampled with 12 accessions from seven wild yam species. A total of 26 accessions were surveyed for restriction fragment length polymorphisms (RFLP) in chloroplast DNA (cpDNA) and nuclear ribosomal DNA (rDNA) using seven restriction endonucleases and various heterologous probes. Chloroplast DNA probes covering 80% of the total chloroplast genome revealed nine restriction site changes and one length mutation among the cpDNAs of Guinea yams and their wild relatives. The estimated numbers of nucleotide substitutions per site (d) among these cpDNAs were very low (0.0005–0.0027), indicating a rather recent divergence of this group. On the basis of these ten mutations, five chloroplast genome types (A-E) were recognized. It was revealed that two cultivated species (D. rotundata and D. cayenensis) display the same chloroplast genome type, type A, as the three wild species D. praehensilis, D. liebrechtsiana and D. abyssinica. Chloroplast genome types B, C, D and E were found in D. minutiflora, D. burkilliana, D. smilacifolia and D. togoensis, respectively. Maximum parsimony analysis produced a hypothetical phylogeny of three primary lineages among cpDNAs of Guinea yams and their relatives: the genome type A lineage, the genome type B lineage and one lineage including genome types C, D and E.
Using rDNA clones of rice and taro as probes, we detected ribosomal DNA variation, presumably at the intergenic spacer region, in Guinea yams and their wild relatives. The survey of rDNA together with that of cpDNA indicates that D. rotundata (white yam) was domesticated from either D. abyssinica, D. liebrechtsiana or D. praehensilis or their hybrid, and that D. cayenensis (yellow yam) is derived from hybridization between a male plant of either D. burkilliana, D. minutiflora or D. smilacifolia and a female plant of either D. rotundata, D. abyssinica, D. liebrechtsiana or D. praehensilis. We propose that the previous nomenclature of white yam should be retained, D. rotundata Poir. nomen nudum, and that yellow yam should be treated as a variety of D. rotundata, denoted as D. rotundata var. x ‘cayenensis’.
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References
Akoroda MO, Chheda HR (1983) Agro-botanical and species relationships of Guinea yams. Trop Agric 60:242–248
Ayensu ES (1972) Dioscoreales. In: Metcalfe CR (ed) Anatomy of the monocotyledons. Oxford University Press, London, pp 1–182
Burkill IH (1960) The organography and the evolution of the Dioscoreaceae, the family of the yams. J Linn Soc London Bot 56:319–412
Chevalier A (1936) Contribution à l'étude de quelques espèces africaines du genre Dioscorea. Bull Mus Natl Hist Nat Bot 8:520–525
Clegg MT, Rawson JR, Thomas K (1984) Chloroplast DNA variation in pearl millet and related species. Genetics 106:449–461
Coursey DG (1967) Yams. Longmans, London
Doebley J, Renfroe W, Blanton A (1987) Restriction site variation in the Zea chloroplast genome. Genetics 117:139–147
Dumont R (1982) Ignames spontanées et cultivées au Benin et en Haute-Volta. In: Miège J, Lyonga SN (eds) Yams ignames. Oxford University Press, Oxford, pp 31–36
FAO (1988) Year production book. FAO, Rome
Hamon P (1988) Structure, origine génétique des ignames cultivées du complexe Dioscorea cayenensis-rotundata et domestication des ignames en afrique de l'ouest. Editions de l'ORSTOM, TDM no. 47, Paris
Hamon P, Toure B (1990) The classification of the cultivated yam (Dioscorea cayenensis-rotundata complex) of West Africa. Euphytica 47:179–187
Hutchinson J, Dalziel JM (1931) Flora of West Tropical Africa, 1st ed, vol II. Crown Agents, London, pp 379–382
Kolodner R, Tewari KK (1975) The molecular size and conformation of the chloroplast DNA from higher plants. Biochem Biophys Acta 402:372–390
Martin FW, Rhodes AM (1978) The relationship of Dioscorea cayenensis and D. rotundata. Trop Agric 55:193–206
Matthews P (1990) The origins, dispersal and domestication of Taro. PhD thesis, Australian National University, Canberra
Miège J (1954) Les cultures vivrieres en Afrique Occidentale. Cah d'outre-mer 7:25–50
Miège J (1968) Dioscoreaceae. In: Hepper FW (ed) Flora of West Tropical Africa, 2nd ed, vol III, part I. Crown Agents, London, pp 144–154
Miège J (1979) Sur quelques problemes taxonomiques posés par Dioscorea cayenensis et D. rotundata. In: Kunkel G (ed) Taxonomic aspects of African economic botany. Proc IXth Plenary Meeting of AETFAT. Las Palmas de Gran Canaria, pp 109–113
Miège J (1982) Appendice. Notes sur les espèces Dioscorea cayenensis Lamk. et D. rotundata Poir. In: Miège J, Lyonga SN (eds) Yams ignames. Oxford University Press, Oxford, pp 367–375
Murray MG, Thompson WF (1980) Rapid isolation of high-molecular-weight plant DNA. Nucleic Acids Res 8:4321–4325
Nei M, Tajima F (1983) Maximum likelihood estimation of the number of nucleotide substitutions from restriction sites data. Genetics 105:207–217
Ogihara Y, Tsunewaki K (1982) Molecular basis of the genetic diversity of the cytoplasm in Triticum and Aegilops. I. Diversity of the chloroplast genome and its lineage revealed by the restriction pattern of ctDNAs. Jpn J Genet 57:371–396
Ogihara Y, Tsunewaki K (1988) Diversity and evolution of chloroplast DNA in Triticum and Aegilops as revealed by restriction fragment analysis. Theor Appl Genet 76:321–332
Onyilagha JC, Lowe J (1985) Studies on the relationships of Dioscorea cayenensis and Dioscorea rotundata cultivars. Euphytica 35:733–739
Oono K, Sugiura M (1978) Isolation of rRNA genes in rice. Jpn J Genet 53:444
Palmer JD, Zamir D (1982) Chloroplast DNA evolution and phylogenetic relationships in Lycopersicon. Proc Natl Acad Sci USA 79:5006–5010
Palmer JD, Shields CR, Cohen DB, Orton TJ (1983) Chloroplast DNA evolution and the origin of amphidiploid Brassica species. Theor Appl Genet 65:181–189
Palmer JD, Jorgensen RA, Thompson WF (1985) Chloroplast DNA variation and evolution in Pisum: Patterns of change and phylogenetic analysis. Genetics 109:195–213
Palmer JD, Jansen RK, Michaels HJ, Chase MW, Manhart JR (1988) Chloroplast DNA variation and plant phylogeny. Ann Missouri Bot Gard 75:721–726
Rogers SO, Bendich AJ (1987) Ribosomal RNA genes in plants: variability in copy number and in the intergenic spacer. Plant Mol Biol 9: 509–520
Sneath PHA, Sokal RO (1973) Numerical taxonomy. Freeman, San Francisco
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517
Terauchi R, Terachi T, Tsunewaki K (1989) Physical map of chloroplast DNA of aerial yam, Dioscorea bulbifera L. Theor Appl Genet 78:1–10
Terauchi R, Terachi T, Tsunewaki K (1991) Intraspecific variation of chloroplast DNA in Dioscorea bulbifera L. Theor Appl Genet 81:461–470
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Terauchi, R., Chikaleke, V.A., Thottappilly, G. et al. Origin and phylogeny of Guinea yams as revealed by RFLP analysis of chloroplast DNA and nuclear ribosomal DNA. Theoret. Appl. Genetics 83, 743–751 (1992). https://doi.org/10.1007/BF00226693
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DOI: https://doi.org/10.1007/BF00226693