Summary
Light microscopic autoradiography performed subsequent to intraocular injection of 3H-leucine revealed silver grains (SG) above axons of the optic tract which could be followed into the ventral and caudal portion of the suprachiasmatic nuclei (SCN) and above the contralateral anterior hypothalamic nucleus (AHN). By high resolution photometric measurement and computer processing the labelled areas were analysed, thus yielding statistical data of the relative grain distribution. The highest SG density was found in the ventrolateral part of both SCN (SCvl), confirming earlier reports concerning retinohypothalamic connections. That area exhibiting a cytoarchitecture different from the remaining nucleus was traversed, however, by numerous labelled axons. In the caudal part of both SCN a specific projection field of retinal fibres could be located. Here, almost no traversing fibres contribute to the rather circumscribed marked area. In the ventral part of the contralateral AHN, diffuse labelling well above background levels could be observed. Distinction between bypassing and terminating fibres within the SCvl could not be made using light microscopy. Analysis of SG distribution of the SCvl with electron microscopic autoradiography revealed a specific localization of SG within presynaptic terminals containing clear vesicles and pale mitochondria.
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Bachmann, L., Salpeter, M.M., Salpeter, E.E.: Das Auflösungsvermögen elektronenmikroskopischer Autoradiographien. Histochemie 15, 234–250 (1968)
Bellonici, J.: Über die zentrale Endigung des Nervus opticus bei den Vertebraten. Zeitschr. für wissensch. Zoologie 47, 1–46 (1888)
Cowan, W.M., Gottlieb, D.I., Hendrickson, A.E., Price, J.L., Woolsey, T.A.: The autoradiographic demonstration of axonal connections in the central nervous system. Brain Res. 37, 21–51 (1972)
Dekker, J.J., Kuypers, H.G.J.M.: Electron microscopy study of forebrain connections by means of the radioactive labeled amino acid tracer technique. Brain Res. 85, 229–235 (1975)
Dendy, P.P.: A method for the automatic estimation of grain densities in microautoradiography. Phys. in Med. y Biol. 5, 131–137 (1960)
Dormer, P.: Auflichtphotometrische Untersuchungen zur Größe der Koinzidenz in der Autoradiographie mit Tritium. Histochemie 8, 1–8 (1967)
Dörmer, P.: Photometric methods in quantitative autoradiography. In: Microautoradiography and Electron Probe Analysis (U. Lüttge, ed), pp. 7–48. Berlin-Heidelberg-New York: Springer 1972
Dörmer, P., Brinkmann, W., Stieber, A., Stich, W.: Automatische Silberkornzählung in der Einzelzell Autoradiographie. Eine neue photometrische Methode für die quantitative Autoradiographie. Klin. Wschr. 44, 477–482 (1966)
Droz, B.: Renewal of synaptic proteins. Brain Res. 62, 383–394 (1973)
Entingh, D.J., Capowski, J.J.: Automatic silver grain counting with area accumulation. Automatic Cytology Conference, Asimolar, Calif. (1975)
Ghetti, B., Horoupian, D.S., Wisniewski, H.M.: Transsynaptic response of the lateral geniculate nucleus and the pattern of degeneration of the nerve terminal in the rhesus monkey after eye enucleation. Brain Res. 45, 31–48 (1972)
Hayhow, W.R., Sefton, A., Webb, C.: Primary optic centers of the rat in relation to the terminal distribution of the crossed and uncrossed optic nerve fibers. J. comp. Neurol. 118, 295–322 (1962)
Hayhow, W.R., Webb, C., Jervie, A.: The accessory optic fiber system in the rat. J. comp. Neurol. 115, 187–216 (1960)
Hendrickson, A.E.: Electron microscopic distribution of axoplasmic transport. J. comp. Neurol. 144, 381–398 (1972)
Kopriwa, B.M., Leblond, C.P.: Improvements in the coating technique of radioautography. J. Histochem. 10, 269–285 (1962)
Lajtha, A.: Transport and incorporation of amino acids in relation to measurement of axonal flow. In: The Use of Axonal Transport for Studies of Neuronal Connectivity (Cowan, W.M. and M. Cuenod, eds.), pp. 26–45. Amsterdam-Oxford-New York: Elsevier 1975
Lipkin, L.E., Lemkin, P., Carman, G.: Automated autoradiographic grain counting in human determined context. J. Histochem. Cytochem. 22, 755–765 (1974)
Mai, J.: Quantitative autoradiographische Untersuchungen am subcorticalen optischen System der Albinoratte. Dissertation, Düsseldorf 1976
McEwen, B.S., Grafstein, B.: Rapid transport of labelled material in fish optic nerve. In: Macromolecules and the Function of the Neuron (Z. Lodin and S.P.R. Rose, eds.), pp. 246–255. Proc. of the Internat. Symp. on metabolism of nuclei acids and proteins and the function of the neuron Prag, 1967. Amsterdam: Ex. Medic. Found 1968
Moore, R.Y., Lena, N.J.: A retinohypothalamic projection in the rat. J. comp. Neurol. 146, 1–14 (1972)
Nadler, N.J.: Interpretation of grain counts in electron microscope radioautography. J. Cell Biol. 49, 877–882 (1971)
Palkovits, M., Zàborszky, L., Ambach, G.: Accessory neurosecretory cell groups in the rat hypothalamus. Acta morph. Acad. Sci. hung. 22 (1), 21–33 (1974)
Rogers, A.W.: A simple photometric device for the quantitation of silver grains in autoradiographs of tissue sections. Exp. Cell. Res. 24, 228–239 (1961)
Rosenstein, J.M., Leure du Pree, A.E.: Electron microscopic observations of nodes of Ranvier in the external cuneate nucleus. J. comp. Neurol. 170, 461–484 (1976)
Salpeter, M.M., Bachmann, L.: Autoradiography with the electron microscope. A procedure for improving resolution, sensitivity and contrast. J. Cell Biol. 22, 469–477 (1964)
Schultz, R.L., Case, N.M.: A modified aldehyde perfusion technique for preventing certain artifacts in electron microscopy of the central nervous system. J. Microsc. 92, 69–84 (1970)
Venable, J.H., Cogeshall, R.: A simpliefied lead citrate stain for use in electron microscopy. J. Cell Biol. 25, 405–408 (1965)
Wann, D.F., Price, J.L., Cowan, W.M., Agulnek, M.A.: An automated system for counting silver grains in autoradiographs. Brain Res. 81, 31–58 (1974)
Weibel, E.R., Fisher, C., Gahm, J., Schaefer, A.: Current capabilities and limitations of available stereological techniques. III. Image analysis with the scanning microphotometer. J. Microsc. 95, 367–392 (1972)
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Mai, J.K., Junger, E. Quantitative autoradiographic light- and electron microscopic studies on the retinohypothalamic connections in the rat. Cell Tissue Res. 183, 221–237 (1977). https://doi.org/10.1007/BF00226621
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DOI: https://doi.org/10.1007/BF00226621