Summary
Satellite cells were traced autoradiographically during the regeneration of skeletal muscle in young Sprague-Dawley rats. Approximately 31% of the satellite cells in uninjured muscles appeared labelled after three injections of tritiated thymidine; none of the myonuclei were labelled in the same muscles. Four to six days after transplanting the radioactive muscles to non-radioactive littermates, regenerating myotube nuclei in the host appeared labelled. Thus, this study confirms that satellite cells in young rats can differentiate into multinucleated myotubes following muscle injury.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Bateson, R.G., Woodrow, D.F., Sloper, J.C.: Circulating cell as a source of myoblasts in regenerating injured mammalian skeletal muscle. Nature (Lond.) 213, 1035–1036 (1967)
Bintliff, S., Walker, B.E.: Radioautographic study of skeletal muscle regeneration. Amer. J. Anat. 106, 223–246 (1960)
Bischoff, R.: Enzymatic liberation of myogenic cells from adult rat muscle. Anat. Rec. 180, 645–662 (1974)
Bischoff, R.: Regeneration of single muscle fibers in vitro. Anat. Rec. 182, 215–236 (1975)
Carlson, B.M.: The regeneration of minced muscles. Monographs in developmental biology, Vol. 4, pp. 117–119. Basel: S. Karger AG 1972
Church, J.C.T., Noronha, R.F.X., Allbrook, D.F.: Satellite cells and skeletal muscle regeneration. Brit. J. Surg. 53, 638–642 (1966)
Dunis, D.A., Namenwirth, M.: The role of grafted skin in the regeneration of X-irradiated skeletal limbs. Devel. 56, 97–109 (1977)
Elyakova, G.V.: Electron microscopic investigation of the formation of myoblasts in regenerating muscular tissue. Dokl. Akad. Nauk SSSR, 202, 1196–1198 (1972) (in Russian)
Hay, E.D.: Electron microscopic observations of muscle dedifferentiation in regenerating Amblystoma limbs. Develop. Biol. 1, 555–585 (1959)
Hay, E.D.: Cellular basis of regeneration. In: Concepts of development. (J. Cash and J.R. Whittaker, eds.), pp. 404–428. Sinaver Assoc., Inc.: Stamford, Connecticut 1974
Jirmanová, I., Thesleff, S.: Ultrastructural study of experimental muscle degeneration and regeneration in the adult rat. Z. Zellforsch. 131, 77–97 (1972)
Kahn, E.B., Simpson, S.B.: Satellite cells in mature, uninjured skeletal muscle of the lizard tail. Develop. Biol. 37, 219–223 (1974)
Kahn, L.B., Meyer, J.S.: Acute myopathy in chronic alcoholism: A study of 22 autopsy cases, with ultrastructural observations. Amer. J. clin. Path. 53, 516-530.
Lentz, T.L.: Cytological studies of muscle dedifferentiation and differentiation during limb regeneration of the newt, Triturus. Amer. J. Anat. 124, 447-480.
Mastaglia, F.L., Dawkins, R.L., Papadimitrou, J.M.: Morphological changes in skeletal muscle after transplantation. A light- and electron-microscopic study of the initial phase of degeneration and regeneration. J. neurol. Sci. 25, 227–247 (1975)
Mauro, A.: Satellite cells of skeletal muscle fibers. J. biophys. biochem. Cytol. 9, 493–495 (1961)
Neerunjun, J.S., Dubowitz, V.: Identification of regenerated dystrophic minced muscle transplanted in normal mice. J. neurol. Sci. 24, 33–38 (1975)
Price, H.M., Howes, E.L., Blumberg, J.M.: Ultrastructural alterations in skeletal muscle fibers injured by cold. I. The acute degenerative changes. Lab. Invest. 13, 1264–1278 (1964b)
Reznik, M.: Origin of myoblasts during skeletal muscle regeneration. Lab. Invest. 20, 353–363 (1969)
Schultz, E.: Fine structure of satellite cells in growing skeletal muscle. Amer. J. Anat. 147, 49–70(1976)
Shafiq, S.A., Gorycki, M.A.: Regeneration in skeletal muscle of mouse: Some electron-microscopic observations. J. Path. Bact. 90, 123–127 (1965)
Snow, M.H.: Myogenic cell formation in regenerating rat skeletal muscle injured by mincing. I. A fine structural study. Anat. Rec. 188, 181–200 (1977a)
Snow, M.H.: Myogenic cell formation in regenerating rat skeletal muscle injured by mincing. II. An autoradiographic study. Anat. Rec. 188, 201–218 (1977b)
Snow, M.H.: The effects of aging on satellite cells in skeletal muscles of mice and rats. Cells Tiss. Res. (Ms 85)
Teräväinen, H.: Satellite cells of striated muscle after compression injury so slight as not to cause degeneration of the muscle fibers. Z. Zellforsch. 103, 320–327 (1970)
Thornton, C.S.: The histogenesis of muscle in the regenerating forelimb of larval Amblystoma punctatum. J. Morph. 62, 17–47 (1938)
Author information
Authors and Affiliations
Additional information
Supported by NIH grant No. 5 S01-RR05356-13
I wish to acknowledge the excellent technical assistance of Ms. Amy Erisman
Rights and permissions
About this article
Cite this article
Snow, M.H. An autoradiographic study of satellite cell differentiation into regenerating myotubes following transplantation of muscles in young rats. Cell Tissue Res. 186, 535–540 (1978). https://doi.org/10.1007/BF00224941
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00224941