Summary
Protoplasts derived from different cytoplasmic male-sterile cultivars of Nicotiana tabacum were fused. Nearly 200 cybrid calli were regenerated into plants and their flower morphologies were examined. Most cybrids exhibited parental-type male-sterile morphologies. Some, however, showed novel male-sterile phenotypes or phenotypes which combined traits of both male-sterile parents in a new combination. Others were restored to fertility, with stamens which produced functional pollen.
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Kofer, W., Glimelius, K. & Bonnett, H.T. Modifications of floral development in tobacco induced by fusion of protoplasts of different male-sterile cultivars. Theoret. Appl. Genetics 79, 97–102 (1990). https://doi.org/10.1007/BF00223793
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DOI: https://doi.org/10.1007/BF00223793