Summary
Eleven F1 hybrid cultivars of cauliflower, representing a range of maturity types, were examined for their responsiveness to anther culture. Embryos were produced from each of the cultivars tested, and the mean embryo yield varied from 82.2 embryos per 100 anthers cultured for cv Dova to 0.6 embryos for cv Serrano. Variation between genotypes and between plants within a genotype was significant, both in terms of embryo yield and percentage responsive anthers. Autumn and winter maturing cauliflowers were generally more responsive than summer types. Embryo yields were enhanced by culturing anthers on solid rather than on liquid media. An increase in concentration of 2,4-Dichlorophenoxyacetic acid (2,4-D) from 0.1 to 0.3 mg/l also increased embryo yield. Embryo yield was doubled when anthers were cultured on solid media containing 0.3 mg/l 2,4-D compared to liquid media containing 0.1 mg/l 2,4-D. Although bud size alone did not have a significant effect on embryo production, genotype x bud size and plant x bud size (within genotype) interactions were significant. Estimation of the variance components demonstrated that, apart from the residual plate-to-plate variation, variation between plants was the largest source of variation, accounting for approximately 30% of total variance. Plant x bud size (within genotype) interaction accounted for 18% of total variance and genotypic differences for approximately 8%.
Article PDF
Similar content being viewed by others
Explore related subjects
Discover the latest articles, news and stories from top researchers in related subjects.Avoid common mistakes on your manuscript.
References
Bagga S, Bhalla-Sarin N, Sopory SK, Guha Mukherjee S (1982) Comparison of in vitro plant formation from somatic tissues and pollen grains in Brassica oleracea var. botrytiss. Phytomorphology 32:152–156
Biddington NL, Sutherland RA, Robinson HT (1988) Silver nitrate increases embryo production in anther culture of Brussels sprouts. Ann Bot 62:181–185
Cardy BJ (1986) Production of anther-derived doubled haploids for breeding oilseed rape (Brassica napus L.). PhD Thesis, University of Guelph
Chiang MS, Frechette S, Kuo CG, Chong C, Delafield ST (1985) Embryogenesis and haploid plant production from anther culture of cabbage. Can J Plant Sci 65:1033–1037
Dunwell JM (1985) Haploid cell cultures. In: Dixon RA (ed) Plant cell culture — A practical approach. ILR Press, Oxford, pp 21–36
Dunwell JM, Cornish M, DeCourcel ACL, Middlefell-Williams JE (1983) Induction and growth of ‘microspore-derived’ embryos of Brassica napus ssp. oleifera. J Exp Bot 34:1768–1778
Keller WA, Armstrong KC (1981) Production of anther-derived dihaploid plants in autotetraploid marrow stem kale (Brassica oleracea var. acephala). Can J Genet Cytol 23:259–265
Keller WA, Armstrong KC (1983) Prodcution of haploids via anther culture in Brassica oleracea var. italica. Euphytica 32:151–159
Keller WA, Rajhathy T, Lacapra J (1975) In vitro production of plants from pollen in Brassica campestris. Can J Genet Cytol 17:655–666
Keller WA, Armstrong KC, Roche de la I (1983) The production and utilization of microspore-derived haploids in Brassica crops. In: Sen SK, Giles KL (eds) Plant cell culture in crop improvement. Plenum Press, New York, pp 169–183
Lichter R (1981) Anther culture of Brassica napus in a liquid culture medium. Z Pflanzenphysiol 103:229–237
Lillo C, Hansen M (1987) Anther culture of cabbage. Influence of growth temperature of donor plants and media composition on embryo yield and plant regeneration. Norw J Agric Sci 1:105–109
Loh CS, Ingram DS (1982) Production of haploid plants from anther cultures and secondary embryoids of winter oilseed rape Brassica napus spp. oleifera. New Phytol 91:507–516
Ockendon DJ (1984) Anther culture in Brussels sprouts (Brassica oleracea var. gemmifera). I. Embryo yields and plant regeneration. Ann Appl Biol 105:285–291
Ockendon DJ (1985) Anther culture in Brussels sprouts (Brassica oleracea var. gemmifera). II, Effects of genotype on embryo yields. Ann Appl Biol 107:101–104
Ockendon DJ (1986) Utilization of anther culture in breeding Brussels sprouts. In: Horn W, Jensen CJ, Odenbach W, Scheider O (eds) Genetic manipulation in plant breeding. de Gruyter, Berlin, pp 265–272
Ockendon DJ (1988) The ploidy of plants obtained from anther culture of cauliflowers. Ann Appl Biol 113:319–325
Ockendon DJ, Sutherland RA (1987) Genetic and non-genetic factors affecting anther culture of Brussels sprouts (Brassica oleracea var. gemmifera). Theor Appl Genet 74:566–570
Orton TJ, Browers MA (1985) Segregation of genetic markers among plants regenerated from anthers of broccoli (Brassica oleracea var. italica). Theor Appl Genet 69:637–643
Pechan PM, Keller WA (1988) Identification of potentially embryogenic microspores in Brassica napus. Physiol Plant 74:377–384
Sovari S (1985) Production of haploids from anther culture in agriculturally valuable Brassica campestris L. cultivars. Ann Agric Fenn 24:149–160
Author information
Authors and Affiliations
Additional information
Communicated by J. W. Snape
Rights and permissions
About this article
Cite this article
Phippen, C., Ockendon, D.J. Genotype, plant, bud size and media factors affecting anther culture of cauliflowers (Brassica oleracea var. botrytis). Theoret. Appl. Genetics 79, 33–38 (1990). https://doi.org/10.1007/BF00223783
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00223783