Summary
Using different genotypes of tomato and diploid potato, possessing alien selectable markers as well as endogenous markers, very high frequencies of protoplast fusion hybrids were obtained. One endogenous genetic marker, the amylose-free (amf) mutant of potato, was helpful not only for the confirmation of fusion products but also for the study of genetic complementation and the segregation of amylose-free starch in microspores. Cytological analysis of the fusion hybrids indicated that except for one which was hexaploid, all of them had a perfectly balanced chromosome number of allotetraploid constitution (2n = 4x = 48). Despite normal chromosome pairing and a diploid behaviour, the microspores in some of the fusion hybrids segregated for the recessive amf-locus. This anomalous segregation of a recessive character in these hybrids was shown not to be due to chromosome elimination or to the absence of the wild-type tomato Amf gene. Although all fusion hybrids were totally sterile, the hexaploid produced stainable pollen and berries with badly developed seeds. Embryo rescue has so far failed to produce backcross progeny.
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Cap GB, Roberts PA, Thomason IJ, Murashige T (1991) Embryo culture of Lycopersicon esculentum x L. peruvianum: hybrid genotypes possessing heat-stable resistance to Meloidogyne incognita. J Am Soc Hort Sci 116:1082–1088
Gebhardt C, Ritter E, Debner T, Schahtschabel U, Walkemier B, Uhrig H, Salamini F (1989) RFLP analysis and linkage mapping in Solanum tuberosum. Theor Appl Genet 78:65–75
Gottschalk W, Peters N (1955) Die Chromosomenstruktur als Kriterium für Abstammungsfragen bei Tomate und Kartoffel. Z Pflanzenzüchtg 34:71–84
Hosticka LP, Hanson MR (1984) Induction of plastid mutations in tomatoes by nitrosomethylurea. J Hered 75:242–246
Hovenkamp-Hermelink JHM, de Vries JN, Adamse P, Jacobsen E, Witholt B, Feenstra WJ (1988) Rapid estimation of the amylose/amylopectin ratio in small amounts of tuber and leaf tissue of the potato. Potato Res 31:241–246
Jacobsen E, Hovenkamp-Hermelink JHM, Krijgsheld HT, Nijdam H, Pijnacker LP, Withold B, Feenstra WJ (1989) Phenotypic and genotypic characterisation of an amylose-free starch mutant of potato. Euphytica 44:43–48
Martin FW (1959) Staining and observing pollen tubes in the styles by means of fluorescence. Stain Tech 34:125–128
Mattheij WM, Eijlander R, de Koning JRA, Louwes KM (1992) Interspecific hybridization between the cultivated potato Solanum tuberosum subspecies tuberosum L. and the wild species L. circaeifolium subspecies circaeifolium. Bitter-exhibiting resistance to Phytophthora infestans (Mont.) de Bary and Globodera pollida (Stone). Behrens. 1. somatic hybrids. Theor Appl Genet 83:459–466
Melchers G (1978a) Potatoes for combined somatic and sexual breeding methods; plants from protoplasts and fusion of protoplasts of potato and tomato. In: Alfermann AW, Reinhard E (eds) Production of natural compounds by cell culture methods. Proc Int Symp Plant Cell Culture, München, pp 306–311
Melchers G (1978b) Plant hybrids by fusion of protoplasts. Proc Symp Plant Tissue Culture. Science Press, Peking, pp 279–283
Melchers G (1979) Protoplast fusion, mechanism and consequences for potato breeding and production of potatoes + tomatoes. In: Advances in protoplast research. Proc 5th Int Protoplast Symp Szeged, Hungary, pp 283–286
Melchers G (1980) The somatic hybrids between tomatoes and potatoes (Topatoes and Pomatoes). In:Sala F, Parisi B, Cella R, Ciferri O (eds) Plant cell cultures: results and perspectives. Elsevier/North Holland Biomedical Press, pp 57–58
Melchers G (1982) The first decennium of somatic hybridization. Proc 5th Int Cong Plant Tissue and Cell Culture, pp 13–16
Melchers G (1984) Topatoes and pomatoes, somatic hybrids between tomatoes and potatoes. In: Röhlic P, Bácsy E (eds) Akademiai Kiadó, Budapest, pp 499–513
Melchers G, Sacristán MD, Holder AA (1978) Somatic hybrid plants of potato and tomato regenerated from fused protoplasts. Carlsberg Res Commun 43:203–218
Menczel L, Nagy F, Kiss ZR, Maliga P (1981) Streptomycin-resistant and sensitive somatic hybrids of Nicotiana tabaccum + Nicotiana knightiana: correlation of resistance to N. tabaccum plastids. Theor Appl Genet 59:191–195
Menczel L, Galiba G, Nagy F, Maliga P (1982) Effect of radiation dosage on efficiency of chloroplast transfer by protoplast fusion in Nicotiana. Genetics 100:487–495
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
Poulson C, Porath D, Sacristán MD, Melchers G (1980) Peptide mapping of the ribulose biphosphate carboxylase small sub-unit from the somatic hybrid of tomato and potato. Carlsberg Res Commun 45:249–267
Ramanna MS, Prakken R (1967) Structure of and homology between pachytene and somatic metaphase chromosomes of the tomato. Genetica 38:115–133
Schiller B, Herrmann RG, Melchers G (1982) Restriction endonuclease analysis of plastid DNA from tomato, potato and some of their somatic hybrids. Mol Gen Genet 186:453–459
Shepard JF, Bidney D, Barsby T, Kemble R (1983) Genetic transfer in plants through intespecific protoplast fusion. Science 219:683–688
Suurs LCJM, Jongedijk E, Tan MMC (1989) Polyacrylamide gradient gel electrophoresis: a routine method for high resolution isozyme electrophoresis of Solanum and Lycopersicon species. Euphytica 40:181–186
Vos-Scheperkeuter GH, de Boer W, Visser RGF, Feenstra WJ, Witholt B (1986) Identification of granule-bound starch synthase in potato tubers. Plant Physiol 82:411–416
Weide R, Koornneef M, Zabel P (1989) A simple non-destructive spraying assay for the detection of an active kanamycinresistance gene in transgenic tomato plants. Theor Appl Genet 78:169–172
Wijbrandi J, van Capelle W, Hanhart CJ, van Loenen, Martinet-Schuringa EP, Koornneef M (1990) Selection and characterization of somatic hybrids between Lycopersicon esculentum and Lycopersicon peruvianum. Plant Sci 70:197–208
Wolters AMA, Schoenmaker HCH, van der Meulen-Muisers JJM, van der Knaap E, Derks FHM, Koornneef M, Zelcer A (1991) Limited DNA elimination from the irradiated potato parent in fusion products of albino Lycopersicon esculentum and Solanum tuberosum. Theor Appl Genet 83:225–232
Yeh B, Peloquin SJ (1965) Pachytene chromosomes of potato (Solanum tuberosum. Group Andigena). Am J Bot 52:1014–1020
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Jacobsen, E., Reinhout, P., Bergervoet, J.E.M. et al. Isolation and characterization of potato-tomato somatic hybrids using an amylose-free potato mutant as parental genotype. Theoret. Appl. Genetics 85, 159–164 (1992). https://doi.org/10.1007/BF00222854
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DOI: https://doi.org/10.1007/BF00222854