Abstract
The storage proteins of 64 F2-derived F6 recombinant inbred lines (RILs) from the bread wheat cross ‘Prinqual’/‘Marengo’ were analyzed. Parents differed at four loci: Gli-B1 (coding for gliadins), Glu-B1 (coding for HMW glutenin subunits), Glu-A3/Gli-A1 (coding for LMW glutenin subunits/gliadins) and Glu-D3 (coding for LMW glutenin subunits). The effect of allelic variation at these loci on tenacity, extensibility and dough strength as measured by the Chopin alveograph was determined. Allelic differences at the Glu-B1 locus had a significant effect on only tenacity. None of the allelic differences at either the Glu-A3/Gli-A1 or Glu-D3 loci had a significant effect on quality criteria. Allelic variation at the Gli-B1 locus significantly affected all of the dough properties. Epistatic effects between some of the loci considered contributed significantly to the variation in dough quality. Additive and epistatic effects each accounted for 15% of the variation in tenacity. Epistasis accounted for 15% of the variation in extensibility, whereas additive effects accounted for 4%. Epistasis accounted for 14% of the variation in dough strength, and additivity for 9%. The relative importance of epistatic effects suggest that they should be included in predictive models when breeding for breadmaking quality.
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Bietz JA, Shepherd KW, Wall JS (1975) Single-kernel analysis of glutenin: use in wheat genetics and breeding. Cereal Chem 52:513–532
Branlard G, Dardevet M (1985a) Diversity of grain protein and bread wheat quality. II. Correlation between high-molecular-weight subunits of glutenin and flour quality characteristics. J Cereal Sci 3:345–354
Branlard G, Dardevet M (1985b) Diversity of grain protein and bread wheat quality. I. Correlation between gliadin bands and flour quality characteristics. J Cereal Sci 3:329–343
Bushuk W, Zillman RR (1978) Wheat cultivar identification by gliadin electrophoregrams. I. Apparatus, method and nomenclature. Can J Plant Sci 58:505–515
Carrillo JM, Roussel M, Qualset CO, Kasarda DD (1990) Use of recombinant imbred lines of wheat for studying the associations of high-molecular-weight glutenin subunits alleles to quantitative traits. 1. Grain yield and quality prediction tests. Theor Appl Genet 79:321–330
Dachkewitch T, Radaelli R, Biancardi AM, Metakovsky EV, Pogna NE (1993) Genetics of gliadins coded for by the group 1 chromosome in the high-quality bread wheat cultivar ‘Neepawa’. Theor Appl Genet 86:389–399
Galili G, Feldman M (1984) Mapping of glutenin and gliadin genes located on chromosome 1B of common wheat. Mol Gen Genet 193:293–298
Gupta RB (1987) Genetic control of LMW glutenin subunits in bread wheat and association with physical dough properties. In: Lasztity L, Bekes F (eds) Gluten proteins. Proc 3rd Gluten Proteins Workshop. Budapest World Scientific, pp 13–19
Gupta RB, Shepherd KW (1988) Low-molecular-weight glutenin subunits in wheat: their variation, inheritance and association with bread-making quality. In: Moller TE, Koebner RMD (eds) Proc 7th Wheat Genet Symp. Bath Press, Bath, UK, pp 943–949
Gupta RB, Singh NK, Shepherd KW (1989) The cumulative effect of allelic variation in LMW and HMW glutenin subunits on dough properties in the progeny of two bread wheats. Theor Appl Genet 77:57–64
Gupta RB, McRitchie F, Shepherd KW, Ellison F (1991) Relative contribution of LMW and HMW glutenin subunits to dough strength and dough stickiness of bread wheat. In: Bushuk W, Tkachuk R (eds) Proc 4th Int Workshop Gluten Proteins. Winnipeg AACC St Paul, MN, pp 71–80
Jackson EA, Holt LM, Payne PI (1983) Characterization of high-molecular-weight gliadin and low-molecular-weight glutenin subunits of wheat endosperm by two-dimensional electrophoresis and the chromosomal localization of their controlling genes. Theor Appl Genet 66:29–37
Khelifi D, Branlard G (1992) The effects of HMW and LMW subunits of glutenin and of gliadins on the technological quality of progeny from four crosses between poor breadmaking quality and strong wheat cultivars. J Cereal Sci 16:195–209
Kolster P, Eeuwijk FA, van Gelder WMJ (1991) Additive and epistatic effects of allelic variation at the high-molecular-weight glutenin subunit loci in determining the bread-making quality of breeding lines of wheat. Euphytica 55:277–285
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Lagudah ES, O'Brien L, Halloran GM (1988) Influence of gliadin composition and high-molecular-weight subunits of glutenin on dough properties in an F3 population of a breadwheat cross. J Cereal Sci 7:33–42
Lawrence GJ, Shepherd KW (1981) Chromosomal location of genes controlling seed protein in species related to wheat. Theor Appl Genet 59:25–31
Lawrence GJ, Moss HJ, Shepherd KW, Wrigley CW (1987) Dough quality of biotypes of eleven Australian wheat cultivars that differ in high-molecular-weight glutenin subunit composition. J Cereal Sci 6:99–101
Marchylo BA, Kruger JE, Hatcher DW (1989) Quantitative reversephase high-performance liquid chromatographic analysis of wheat storage proteins as a potential quality prediction tool. J Cereal Sci 9:113–130
Moonen JHE, Scheepstra A, Graveland A (1982) Use of SDS-sedimentation test and SDS-polyacrylamide gel electrophoresis for screening breeder's samples of wheat for bread-making quality. Euphytica 31:677–690
Payne PI (1986) Varietal improvement in the bread-making of wheat: contributions from biochemistry and genetics, and future prospects from molecular biology. BCPP Mono 34, Biotechnology and crop improvement and protection, pp 69–81
Payne PI, Lawrence GJ (1983) Catalogue of alleles for the complex loci, Glu-A1, Glu-B1 and Glu-D3 which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res Commun 11:29–35
Payne PI, Law CN, Mudd EE (1980) Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor Appl Genet 58:113–120
Payne PI, Holt LM, Law CN (1981) Structural and genetical studies on the high-molecular-weight subunits of wheat gluten. Part I: Allelic variation in subunits amongst varieties of wheat (Triticum aestivum). Theor Appl Genet 60:229–236
Payne PI, Holt LM, Worland AJ, Law CN (1982) Structural and genetical studies on the high-molecular-weight subunits of wheat. Part 3. Telocentric mapping of the subunit genes on the long arms of homoeologous group 1 chromosomes. Theor Appl Genet 63:129–138
Payne PI, Holt LM, Jackson EA, Law CN (1984) Wheat storage proteins. Their genetics and their potential for manipulation by plant breeding. Philos Trans R Soc London Ser B 304:359–371
Payne PI, Seekings JA, Worland AJ, Jarvis MG, Holt LM (1987a) Allelic variation of glutenin subunits and gliadins and its effect on breadmaking quality in wheat: analysis of F5 progeny from ‘Chinese Spring’ x ‘Chinese Spring’ (Hope 1A). J Cereal Sci 6:103–118
Payne PI, Holt LM, Harinder K, MaCartney DP, Lawrence GJ (1987b) The use of near-isogenic lines with different HMW glutenin subunits in studying bread-making quality and glutenin structure. In: Lasztity L, Bekes F (eds) Gluten proteins. Proc 3rd Gluten Protein Workshop. Budapest World Scientific, pp 216–226
Pogna NE, Autran JC, Mellini F, Lafiandra D, Feillet P (1990) Chromosome 1-B-encoded gliadins and glutenin subunits in durum wheat: genetics and relationship to gluten strenth. J Cereal Sci 9:16–34
Radaelli R, Pogna NE, Darchkevitch T, Cacciatori P, Biancardi AM, Matakovsky EV (1992) Inheritance studies of the 1AS/IDS chromosome translocation in the bread wheat variety ‘Perzivan-1’. J Genet Breed 46:253–262
Rousset M, Carrillo JM, Qualset CO, Kasarda DD (1992) Use of recombinant imbred lines of wheat for study the associations of high-molecular-weight glutenin subunit alleles to quantitative traits. 2. Milling and bread-baking quality. Theor Appl Genet 83:403–412
Singh NK, Shepherd KW (1988) Linkage mapping of genes controlling endosperm storage proteins in wheat. 1. Genes on the short arms of group 1 chromosomes. Theor Appl Genet 75:628–641
Singh NK, Shepherd KW, Cornish GB (1991) A simplified SDS-PAGE procedure for separating LMW subunits of glutenin. J Cereal Sci 14:203–208
Sozinov AA, Poperelya FA (1980) Genetic classification of prolamines and its use for plant breeding. Ann Technol Agric 29:229–245
Wall JS (1979) The role of wheat proteins in determining baking quality. In: Laidman DL, Wyn Jones RG (eds) Recent advances in the biochemistry of cereals. Academic Press, London New York, pp 275–311
Woychik JH, Boundy JA, Dimler RJ (1961) Starch gel electrophoresis of wheat gluten proteins with concentrated urea. Arch Biochem Biophys 94:477–482
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Nieto-Taladriz, M.T., Perretant, M.R. & Rousset, M. Effect of gliadins and HMW and LMW subunits of glutenin on dough properties in the F6 recombinant inbred lines from a bread wheat cross. Theoret. Appl. Genetics 88, 81–88 (1994). https://doi.org/10.1007/BF00222398
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DOI: https://doi.org/10.1007/BF00222398