Summary
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1.
The oenocytes of Gryllus bimaculatus are characterized by an abundant smooth-surfaced ER (ATER). In spite of the great cell size the plasma membrane never shows extensive infoldings during the moulting cycle. In addition to mitochondria there are very large numbers of microbodies containing peroxidase but apparently not uricase. Within the second part of the instar the microbodies lie along the clefts which run through the whole cell.
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2.
The following changes are observed in the course of a moulting cycle: Immediately after hatching the ATER is scarcely developed, some liposomes are located within areas of ATER disappearing some hours later. 20 hours after emergence glycogen deposits appear in two forms: large deposits reaching some μm in diameter, and in distinct rosettes dispersed between the tubules of the ATER. 30 hours post-moult profiles of RER appear, which disappear together with the large glycogen deposits one day later. At the same time ATER is increased and the clefts develop within areas of elongate granules smaller than ribosomes. The number of such clefts subsequently becomes reduced probably as a result of confluence. Towards the end of the moulting cycle numerous autophagosomes appear. These digest parts of the agranular reticulum, many microbodies, and to a lesser extent, mitochondria. Residual bodies are extruded during moult whereas the clefts remain.
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3.
The ultrastructural features parallel those of steroid-producing cells in vertebrates. Besides this it is possible that oenocytes also engage in detoxication processes as shown for vertebrate liver.
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References
Aoki, A.: Hormonal control of Leydig cell differentiation. Protoplasma (Wien) 71, 209–225 (1970)
Baillie, A. H.: Further observations on the growth and histochemistry of the Leydig tissue in the postnatal prepubertal mouse tissue. J, Anat. (Lond.) 98, 403–419 (1964)
Beaulaton, J. A.: Modifications ultrastructurales des cellules sécrétrices de la glande prothoracique de vers à soie au cours des deux derniers âges larvaires. I. Le chondriome, et ses relations avec reticulum agranulaire. J. Cell Biol. 39, 501–525 (1968)
Berchtold, J. P.: Contribution à l'étude ultrastructurale des cellules interrénales de Salamandra salamandra L. (Amphibien Urodèle). I. Conditions normales. Z. Zellforsch. 102, 367–375 (1969)
Blanchette, E. J.: Ovarian steroid cells. I. Differentiation of the lutein cell from the granulosa follicle cell during preovulatory stage and under the influence of exogenous gonadotropins. J. Cell Biol. 31, 501–516 (1966a)
Blanchette, E. J.: Ovarian steroid cells. II. The lutein cell. J. Cell Biol. 31, 517–542 (1966b)
Bolander, R. P., Weibel, E. R.: A morphometric study of the removal of phenobarbital-induced membrane from hepatocytes after cessation of treatment. J. Cell Biol. 56, 746–761 (1973)
Brökelmann, J.: Über die Stütz- und Zwischenzellen des Froschhodens während des spermatogenetischen Zyklus. Z. Zellforsch. 64, 429–461 (1964)
Cassier, P., Fain-Maurel, E. A.: Sur l'abondance d'un reticulum endoplasmique agranulaire et tubulaire dans les oenocytes de Locusta migratoria migratorioides (R. et F.). C. R. Acad. Sci. (Paris) 269, 1979–1981 (1969)
Cassier, P., Fain-Maurel, E. A.: Caractères infrastructuraux et cytochimiques des oenocytes de Locusta migratoria migratorioides en rapport avec les mues et les cycles ovariens. Arch. Anat. micr. Morph. exp. 61, 357–380 (1973)
Christensen, A. K.: The fine structure of testicular interstitial cells in guinea pigs. J. Cell Biol. 26, 911–934 (1965)
Christensen, A. K., Fawcett, D. W.: The fine structure of testicular interstitial cells in mice. Amer. J. Anat. 118, 551–572 (1966)
Clark, M. K., Dahm, P. A.: Phenobarbital-induced, membrane-like scrolls in the oenocytes of Musca domestica Linnaeus. J. Cell Biol. 56, 870–875 (1973)
Day, M. F.: The function of the corpus allatum in muscoid Diptera. Biol. Bull. 84, 127–140 (1943)
Delachambre, M. J.: Remarques sur l'histophysiologie des oenocytes épidermiques de la nymphe de Tenebrio molitor L. C. R. Acad. Sci. (Paris) 263, 764–767 (1966)
De Duve, Ch., Baudhuin, P.: Peroxisomes (Microbodies and related particles) Physiol. Rev. 46, 323–357 (1966)
De Duve, Ch., Wattiaux, R.: Functions of lysosomes. Ann. Rev. Physiol. 28, 435 (1966)
Dorn, A.: Die endokrinen Drüsen im Embryo von Oncopeltus fasciatus Dallas (Insecta, Heteroptera). Morphogenese, Funktionsaufnahme, Beeinflussung des Gewebewachstums und Beziehungen zu den embryonalen Häutungen. Z. Morph. Tiere 71, 52–104 (1972)
Dufaure, J. P.: L'ultrastructure du testicule de lézard vivipare (Reptile, Lacertilien). II. Les cellules de Sertoli. Étude du glykogène. Z. Zellforsch. 115, 565–578 (1971)
Evans, J. T.: Development and ultrastructure of the fat body cells and oenocytes of the Queensland fruit fly Dacus tryoni (Frogg). Z. Zellforsch. 81, 49–61 (1967)
Frank, A. L., Christensen, A. K.: Localization of acid phosphatase in lipofuscin granules and possible autophagic vacuoles in interstitial cells of guinea pig testis. J. Cell Biol. 36, 1–13 (1968)
Gnatzy, W.: Struktur und Entwicklung des Integuments und der Oenocyten von Culex pipiens L. (Dipt.). Z. Zellforsch. 110, 401–443 (1970)
Harmsen, R., Beckel, W. E.: The intraovular development of the subspiracular glands in Hyalophora cecropia (L.) (Lepidoptera, Saturnidae). Canad. J. Zool. 38, 883–893 (1960)
Hruban, Z., Rechcigl, M.: Microbodies and related particles. New York: Academic Press, Inc. 1969
Huber, M.: Histologische und experimentelle Untersuchungen über die Oenocyten der Larve von Sialis lutaria L. Z. Zellforsch. 49, 661–697 (1958)
Idelman, S.: Mitochondries et liposomes; description d'une transformation mitochondriale observée dans la cortico-surrénale du rat. J. Microscopie 3, 437–446 (1964)
Karlson, P., Bode, C.: Die Inaktivierung des Ecdysons bei der Schmeißfliege Calliphora erythrocephala MEIGEN. J. Insect Physiol. 15, 111–118 (1969)
Karlson, P., Shaaya, E.: Der Ecdysontiter während der Insektenentwicklung. — I. Eine Methode zur Bestimmung des Ecdysongehalts. J. Insect Physiol. 10, 797–804 (1964)
Krishnakumaran, A., Oberlander, H., Schneidermann, A. H.: Rates of DNA and RNA synthesis in various tissues during larval moult cycle of Samia cynthia ricini (Lepidoptera). Nature (Lond.) 205, 1131–1133 (1965)
Lindner, E.: Die Sacculi mitochondriales der Diskochondrien und Sphaerochondrien in der Nebenniere vom Igel, Erinaceus europaeus L. Z. Zellforsch. 72, 212–235 (1966)
Locke, M.: The ultrastructure of oenocytes in molt/intermolt cycle of an insect. Tissue and Cell. 1, 103–154 (1969)
Locke, M., McMahon, J. T.: The origin and fate of microbodies in the fat body of an insect. J. Cell Biol. 48, 61–78 (1971)
Miller, F., Palade, G. E.: Lytic activities in renal protein absorption droplets. J. Cell Biol. 23, 519–552 (1964)
Moses, H., Davis, W. W., Rosenthal, A. S., Garren, L. D.: Adrenal cholesterol: Localization by electron-microscope autoradiography. Science 163, 1203–1205 (1969)
Pflugfelder, O.: Entwicklungsphysiologie der Insekten. Leipzig: Akad. Verl.-Gesellschaft 1952
Reddy, J., Svoboda, D.: Microbodies (Peroxisomes) in the interstitial cells of rodent testes. Lab. Invest. 26, 657–665 (1972)
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–213 (1963)
Rinterknecht, E., Porte, A., Joly, P.: Contribution à l'étude ultrastructurale de l'oenocyte chez Locusta migratoria. C. R. Acad. Sci. (Paris) 269, 2121–2124 (1969)
Romer, F.: Häutungshormone in den Oenocyten des Mehlkäfers. Naturwissenschaften 58, 324–325 (1971a)
Romer, F.: Die Prothorakaldrüsen der Larve von Tenebrio molitor L. (Tenebrionidae, Coleoptera) und ihre Veränderungen während eines Häutungszyklus. Z. Zellforsch. 122, 425–455 (1971b)
Romer, F.: Veränderungen des Integuments von Gryllus bimaculatus (Saltatoria) während des 1. Larvenstadiums. Z. Naturforsch. 26b, 1386–1388 (1971c)
Romer, F.: Histologie, Histochemie, Polyploidie und Feinstruktur der Oenocyten von Gryllus bimaculatus (Saltatoria). Cytobiologie 6, 195–213 (1972)
Romer, F.: Feinstrukturelle Merkmale der Oenocyten pterygoter Insekten. Verh. dtsch. zool. Ges. 66, 65–70 (1973)
Romer, F., Emmerich, H., Nowock, A.: Biosynthesis of moulting hormones in isolated prothoracic glands and oenocytes of Tenebrio molitor in vitro. In press
Roth, T. F., Porter, K. R.: Yolk protein uptake in the oocyte of the mosquito Aedes aegypti L. J. Cell Biol. 20, 313–332 (1964)
Schneidermann, H. A., Gilbert, L. I.: Control of growth and development in insects. Science 143, 325–333 (1964)
Schmidt, G.: Sekretionsphasen und cytologische Beobachtungen zur Funktion der Oenocyten während der Puppenphase verschiedener Kasten und Geschlechter von Formica polyctena FOERST. (Ins. Hym. Form.). Z. Zellforsch. 55, 707–723 (1961)
Stein, O., Stein, Y., Goodman, D. S., Fidge, N. H.: The metabolism of chylomicron cholesteryl ester in rat liver. A combined radioautographic electron microscopic and biochemical study. J. Cell Biol. 43, 410–431 (1969)
Vogt, M.: Fettkörper und Oenocyten der Drosophila nach Exstirpation der adulten Ringdrüse. Z. Zellforsch. 34, 160–164 (1948)
Weir, B. S.: Control of moulting in insects. Nature (Lond.) 228, 580–581 (1970)
Wigglesworth, V. B.: The physiology of the cuticle and of ecdysis in Rhodnius prolixus (Triatom., Hemipt.) with special reference to the function of the oenocytes and of dermal glands. Quart. J. micr. Sci. 76, 269–318 (1933)
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The work was supported by grants of the Deutsche Forschungsgemeinschaft.
I want to thank Mrs. M. Ullmann and Miss B. Bauke for phototechnical assistance and Mrs. G. Eder for drawing the diagrams.
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Romer, F. Ultrastructural changes of the oenocytes of Gryllus bimaculatus DEG (Saltatoria, Insecta) during the moulting cycle. Cell Tissue Res. 151, 27–46 (1974). https://doi.org/10.1007/BF00222032
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DOI: https://doi.org/10.1007/BF00222032