Summary
Black cuticles of larvae and pupae from various Lepidoptera were studied by light and electron microscopy. There are striking differences in the representation of black pigmentation, especially at the ultrastructural level. Two types may be described: 1. With the light microscope black melanin-like grana, electron-dense electron microscopically, are found in the distal parts of the exocuticle. This type is demonstrated in larvae of Celerio euphorbiae, Papilio machaon, and Phalera bucephala. 2. With the light microscope a dark homogeneous layer in the distal exocuticle can be recognized, however, electron microscopically no structures correlated with this dark pigment layer. This type of pigmentation was present in pupae of Pieris brassicae and Aglais urticae; in Pieris larvae the dark pigmented layer appeared to be limited to the epicuticle. In Celerio processes of the epidermal cells are involved in transporting precursors to the exocuticle. The conclusion was reached that black pigmentation in cuticles is based on different mechanisms as proposed by structural features. The two likely mechanisms are melanization and sclerotization.
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Andersen, S.O.: Phenolic compounds isolated from insect hard cuticle and their relationship to the sclerotization process. Insect Biochem. 1, 157–170 (1971)
Andersen, S.O., Barrett, F.M.: The isolation of the ketocatechols from insect cuticle and their possible role in sclerotization. J. Insect Physiol. 17, 69–83 (1971)
Antoni, A. de, Allergi, G., Costa, C., Bordin, F.: Melanogenesis from tryptophan. Biogenetic experiments with hardiny-passey mouse melanoma. Experientia (Basel) 30, 600–601 (1974)
Barbier, R.: Origine et formation des granules pigmentaires de la cuticle larvaire de Tyria jacobeae. C. R. Acad. Sci. (Paris) D 274, 1839–1842 (1972)
Byers, J.R.: Tyndall blue and surface white of tent caterpillars, Mallacosoma SPP. J. Insect Physiol. 21, 401–415 (1975)
Fogal, W., Fraenkel, G.: The role of bursicon in melanization and endocuticle formation in the adult fleshfly, Sarcophaga bullata. J. Insect Physiol. 15, 1235–1247 (1969)
Fogal, W., Fraenkel, G.: Histogenesis of the cuticle of the adult flies Sarcophaga bullata and S. argyrostoma. J. Morph. 130, 137–146 (1970)
Fuzeau-Braesch, S.: Présence de granules pigmentaires cuticulaires chez la chenille de Tyria jacobae (Lépidoptères) C. R. Acad. Sci. (Paris) D 270, 512–514 (1970)
Fuzeau-Braesch, S.: Pigments and color changes. Ann. Rev. Entomol. 17, 403–424 (1972)
Hackman, R.H.: Melanin in an insect, Lucilia cuprina (Wied). Nature (Lond.) 216, 163 (1967)
Klaus, S.N.: Pigment transfer in mammalian epidermis. Arch. Derm. 100, 756–762 (1969)
Mills, R.R., Fox, F.R.: The sclerotization process by the American cockroach: contribution of melanin. Insect Biochem. 2, 23–28 (1972)
Needham, A.E.: The significance of zoochromes. Berlin-Heidelberg-New York: Springer 1974
Nicolaus, R.A.: Melanins. Paris: Hermann 1968
Post, L.C.: Bursicon: Its effect on tyrosine permeation into insect haemocytes. Biochim. biophys. Acta. (Amst.) 290, 424–428 (1972)
Richards, A.G.: Sclerotization and the localization of brown and black colors in insects. Zool. Jb. Anat. 84, 25–62 (1967)
Vuillaume, M.: Les pigments des Invertébrés. Paris: Masson et Cie. 1969
Wohlfarth-Bottermann, K.E.: Die Kontrastierung tierischer Zellen und Gewebe im Rahmen ihrer elektronenmikroskopischen Untersuchungen an ultradünnen Schnitten. Naturwissenschaften 44, 287–288 (1957)
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This work was supported by the Deutsche Forschungsgemeinschaft (SFB 87, project A1, granted to Prof. Bückmann)
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Kayser-Wegmann, I. Differences in black pigmentation in lepidopteran cuticles as revealed by light and electron microscopy. Cell Tissue Res. 171, 513–521 (1976). https://doi.org/10.1007/BF00220242
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DOI: https://doi.org/10.1007/BF00220242