Summary
Intact and denervated extensor digitorum longus (EDL) muscles of 20-day-old inbred Lewis-Wistar rats were labelled with 3H-thymidine. Ninety minutes after the injection of the isotope 4.0% of the nuclei were labelled in the intact (i.e. innervated) and 9.6% in the muscles, denervated 3 days before administration of the isotope. The labelled EDL muscles were grafted into the bed of the previously removed EDL muscles of inbred animals and these isografts were studied 30 days later.
In the EDL muscles, regenerated from innervated isografts only occasionally labelled endothelial cells were found whereas in the muscles regenerated from denervated isografts also parenchymal muscle nuclei were regularly labelled. The incidence of labelled nuclei in the regenerated EDL muscles was, however, about 20 times lower than in the donor EDL muscles. The present experiments provide a direct proof of utilization of donor satellite cell nuclei for regeneration in grafted muscle tissue. With respect to the low incidence of labelled nuclei in regenerated EDL muscles, other sources of cells apparently also contribute to the regeneration process.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Aloisi, M., Mussini, I., Schiaffino, S.: Activation of muscle nuclei in denervation and hypertrophy. In: Basic research in myology. (B.A. Kakulas, ed.) Excerpta Medica — Int. Congr. Series 294, 338–342 (1973)
Blunt, R.J., Jones, R., Vrbová, G.: The use of local anaesthetics to produce prolonged motor nerve blocks in the study of denervation hypersensitivity. Pflügers Arch. 355, 189–204 (1975)
Carlson, B.M.: The regeneration of skeletal muscle — a review. Am. J. Anat. 137, 119–150 (1973)
Carlson, B.M., Gutmann, E.: Transplantation and “cross” transplantation of free muscle grafts in the rat. Experientia (Basel) 30, 1292–1294 (1974)
Church, J.C.T.: Cell populations in skeletal muscle after regeneration. J. Embryol. exp. Morph. 23, 531–537 (1970)
Gutmann, E.: Factors affecting recovery of motor function after nerve lesions. J. Neurol. Psychiat. 5, 81–95 (1942)
Gutmann, E., Čihák, R., Mareš, L., Rohliček, V.: Facilitation of regeneration of muscle tissue induced by minced liver tissue. Physiol. bohemoslov. 1976 (in press)
Gutmann, E., Young, J.Z.: The reinnervation of muscle after various periods of atrophy. J. Anat. (Lond.) 78, 15–43 (1944)
Hanzlíková, V., Macková, E.V., Hník, P.: Satellite cells of the rat soleus muscle in the process of compensatory hypertrophy combined with denervation. Cell. Tiss. Res. 160, 411–421 (1975)
Hay, E.D.: Cytological studies of dedifferentiation and differentiation in regenerating amphibian limbs. In: Regeneration (D. Rudnick, ed.), p. 177–210. N.J.: The Ronald Press Co. 1962
Hess, A., Rossner, S.: The satellite cell bud and myoblast in denervated mammalian muscle fibers. Amer. J. Anat. 129, 21–40 (1970)
Lee, J.C.: Electron microscope observations on myogenic free cells of denervated skeletal muscle. Exp. Neurol. 12, 123–135 (1965)
Levander, G.: Induction phenomena in tissue regeneration. Baltimore: Williams and Wilkins 1964
Mauro, A.: Satellite cells of skeletal muscle fibers. J. biophys. biochem. Cytol. 9, 493–495 (1961)
Moss, F.P., Leblond, C.P.: Satellite cells as the source of nuclei in muscles of growing rats. Anat. Rec. 170, 421–436 (1971)
Polezhaev, L.V.: Metaplasia and induction of tissues according to Levander (Russian). In: The Metaplasia of Tissues (Russian). (M.S. Mitskewitch, O.G. Stroeva and V.I. Mitashov, eds.). p. 45–73. Moscow: Izdat. Nauka (1970)
Reznik, M.: Satellite cells, myoblasts and skeletal muscle regeneration. In: Regeneration of striated muscle and myogenesis. (eds., A. Mauro, S.A. Shafiq and A.T. Milhorat) p. 133–156. Amsterdam: Excerpta Medica Int. Congress Series, No. 218, 1970
Shafiq, S.A., Goryski, M.A., Milhorat, A.T.: An electron microscopy study of regeneration and satellite cells in human muscle. Neurology 17, 567–575 (1967)
Steen, T.P.: Cell differentiation during salamander limb regeneration. In: Regeneration of Striated Muscle and Myogenesis. (eds. A. Mauro, S.A. Shafiq and A.T. Milhorat), p. 73–90. Amsterdam: Excerpta Medica Int. Congress Series, No. 218, 1970
Studitsky, A.N.: Experimental surgery of muscles. (Russian). Moscow: Izdat. Akad. Nauk, SSSR. (1959)
Teräväinen, H.: Satellite cells of striated muscle after compression injury so slight as not to cause degeneration of the muscle fibers. Z. Zellforsch. 103, 320–327 (1970)
Vyskočil, F., Carlson, B.M., Gutmann, E.: Changes in resting membrane potential and contractility of innervated and denervated skeletal muscle free grafts in the rat. Pflügers Arch. 344, 181–186 (1973)
Walker, B.E.: Skeletal muscle regeneration in young rats. Amer. J. Anat. 133, 369–178 (1972)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gutmann, E., Mareš, V. & Štichová, J. Fate of 3H-thymidine labelled myogenic cells in regeneration of muscle isografts. Cell Tissue Res. 167, 117–123 (1976). https://doi.org/10.1007/BF00220163
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00220163