Summary
Morphology and ultrastructure of osteoid-osteocytes were studied in serial thin sections (700–800 Å thick) of periosteal woven bone in tibiae of 15-day-old chick embryos. The three-dimensional shapes of 21 partially, and of one fully sectioned cell were reconstructed manually and by means of a computer-assisted image analyser.
Osteoid-osteocytes are active cells engaged in organic matrix secretion and calcification. Like osteoblasts, their activity seems to be polarized towards the mineralization front, as shown by the presence of cytoplasmic processes on their mineral-facing side and by the position of the nucleus toward the vascular side of the cytoplasm. Cellular processes directed towards blood vessels appear only at a later stage, i.e. when the mineralization starts to spread all round the cell.
The asynchrony in formation, together with the observed differences in morphology suggest the hypothesis that the cellular processes of the mineral-facing side are mainly involved in bone formation and those of the vascular side in cell nutrition.
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References
Ascenzi A (1964) The relationship between mineralization and bone matrix. In: Blackwood HJJ (ed) Bone and tooth. Pergamon Press, Oxford, pp 231–243
Ascenzi A, Francois C, Bocciarelli SD (1963) On the bone induced by estrogen in birds. J Ultrastruct Res 8:491–505
Bonucci E (1971) Problemi attuali attinenti all'istochimica di talune matrici calcificanti normali e patologiche. Riv Istoch Norm Pat 17:153–234
Bordier PJ, Marie P, Miravet L, Ryckewaert A, Rasmussen H (1976) Morphological and morphometrical characteristics of the mineralization front. A vitamin D regulated sequence of bone remodeling. In: Meunier PJ (ed) Bone Histomorphometry. Armour Montagu, Paris, pp 335–354
Cameron DA (1972) The Ultrastructure of bone. In: Bourne GH (ed) The biochemistry and physiology of bone. Academic Press, New York, pp 191–236
Cooper R, Milgram JW, Robinson RA (1966) Morphology of the osteon. An electron microscopic study. J Bone Joint Surg 48-A: 1239–1271
Decker JD (1966) An electron microscopic investigation of osteogenesis in the embryonic chick. Am J Anat 118:591–614
Dudley HR, Spiro D (1961) The fine structure of bone cells. J Biophysic Biochem Cytol 11:627–649
Gegenbaur C (1864) Über die Bildung des Knochengewebes I u. II. Z Naturwiss Jena 1:343–369
Hancox NM, Boothroyd B (1965) Electron microscopy of the early stages of osteogenesis. Clin Orthop 40: 153–161
Jones SJ (1974) Secretory territories and rate of matrix production of osteoblasts Calc. Tissue Res 14:309–315
Jones SJ, Boyde A (1976) Is there a relationship between osteoblasts and collagen orientation in bone? Isr J Med Sci 12:10–19
Jones SJ, Boyde A, Pawley GB (1975) Osteoblasts and collagen orientation. Cell Tissue Res 159:73–80
Jones SJ, Boyde A, Ness AR (1977) SEM studies of osteoblasts: size, shape and anisotropy in relation to hormonal status in organ culture. In: Meunier PJ (ed) Bone histomorphometry. Armour Montagu, Paris, pp 275–289
Marotti G (1976) Decrement in volume of osteoblasts during osteon formation and its effect on the size of the corresponding osteocytes. In: Meunier PJ (ed) Bone histomorphometry. Armour Montagu, Paris, pp 385–397
Marotti G, Remaggi F, Zaffe D (1985) Quantitative investigation of osteocyte canaliculi in human compact and spongy bone. Bone 6:335–337
Nijweide PJ, van der Plas A, Scherft JP (1981) Biochemical and histological studies on various bone cell preparation. Calcif Tissue Int 33:529–540
Ornoy A, Atkin I, Levy J (1980) Ultrastructural studies on the origin and structure of matrix vesicles in bone of young rats. Acta Anat 106:450–461
Owen M (1963) Cell population kinetics of an osteogenic tissue. J Cell Biol 19:19–32
Pritchard JJ (1972) The osteoblast. In: Bourne GH (ed) The biochemistry and physiology of bone. Academic Press, New York, pp 21–43
Rasmussen H, Bordier P (1974) The physiological and cellular basis of metabolic bone disease. William and Wilkins Company, Baltimore
Remaggi F, Canè V, Muglia MA, Zaffe D (1981) Primi dati sulla densità dei canalicoli emergenti da lacune osteocitarie in osteoni secondari dell'uomo. Arch Ital Anat Embriol [Suppl] 86, p 215
Remaggi F, Zaffe D (1982) Dati quantitativi sull'estensione della rete canalicolare osteocitaria nei tessuti ossei a fibre intrecciate e lamellare dell'uomo in condizioni normali. In: Riassunti XXXVIII Convegno Nazionale Soc Ital Anat. Gruppo Editoriale Medico, Roma, pp 178–179
Waldeyer W (1865a) Über den Ossifikationsprozeß. Arch Mikrosk Anat 1:354–375
Waldeyer W (1865b) Über den Ossifikationsprozeß. Zentralbl Med Wiss 6:113–116
Zylberberg L, Castanet J (1985) New data on the structure and growth of the osteoderms in the Reptilia Anguis fragilis L. (Anguidae, Squamata). J Microsc 186:327–342
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Palumbo, C. A three-dimensional ultrastructural study of osteoid-osteocytes in the tibia of chick embryos. Cell Tissue Res. 246, 125–131 (1986). https://doi.org/10.1007/BF00219008
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DOI: https://doi.org/10.1007/BF00219008