Summary
In order to study the distribution of neuropeptide Y-like immunoreactivity in the human hypothalamus, an immunocytochemical localization of this peptide was performed. Using antibodies developed against synthetic porcine neuropeptide Y (NPY), we have been able to localize immunoreactivity in neuronal cell bodies located exclusively in the infundibular nucleus. Immunostained fibers were found in several regions in the hypothalamus with a high concentration in the periventricular areas. Fibers were also found in the neurovascular zone of the median eminence, the pituitary stalk and the posterior pituitary. These results suggest that immunoreactive material related to porcine NPY is present in the human hypothalamus, with a distribution similar to that observed in the rat.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Adrian TE, Allen JM, Bloom SR, Ghatei MA, Rossor MN, Roberts GW, Crow TJ, Tatemoto K, Polak JM (1983) Neuropeptide Y distribution in human brain. Nature 306:584–586
Allen YS, Adrian TE, Allen JM, Tatemoto K, Crow TJ, Bloom SR, Polak JM (1983) Neuropeptide Y distribution in the rat brain. Science 221:877–879
Benoît R, Bohlen P, Ling N, Briskin A, Esch F, Brazeau P, Ying SY, Guillemin R (1982) Presence of somatostatin-28 (1–12) in hypothalamus and pancreas. Proc Natl Acad Sci (USA) 79:917–921
Daniel PM, Prichard MMJ (1975) Studies of the hypothalamus and the pituitary gland. Acta Endocrinol (Copenh) Suppl 201:1–26
Guy J, Allen YS, Polak JM, Pelletier G (1983) Immunocytochemical localization of neuropeptide Y (NPY) in the rat brain. Proc Soc Neurosci 9, abst. 85.5
Pelletier G, Guy J, Allen YS, Polak JM (1984) Electron microscope immunocytochemical localization of neuropeptide Y (NPY) in the rat brain. Neuropeptides (in press)
Sternberger LA (1972) Immunocytochemistry, Prentice-Hall Inc. Engelwood Cliffs, NJ
Tatemoto K (1982) Neuropeptide Y: complete amino acid sequence of the brain peptide. Proc Natl Acad Sci (USA) 79:5485–5489
Tatemoto K, Carlquist M, Mutt V (1982) Neuropeptide Y-a novel brain peptide with structural similarities to peptide YY and pancreatic polypeptide. Nature 256:659–660
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pelletier, G., Desy, L., Kerkerian, L. et al. Immunocytochemical localization of neuropeptide Y (NPY) in the human hypothalamus. Cell Tissue Res. 238, 203–205 (1984). https://doi.org/10.1007/BF00215163
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00215163