Abstract
The effect of antidiuretic hormone on transepithelial Na+ and Cl- transport and its modulation by aldosterone (10-6 m) was studied in the Xenopus laevis distal nephron cell line A6-C1 by measuring transepithelial electrophysiological parameters and bidirectional anion fluxes. Vasotocin (or vasopressin) induced a biphasic increase in transepithelial short-circuit current (I sc). Early and late effects were potentiated by aldosterone and could be mimicked by forskolin and BrcAMP, implicating cAMP as a mediator. The early increase in I sc (maximum 1–2 min after hormone addition) was resistant to 50 μm amiloride. Electrophysiological experiments with apical ion substitutions or basolateral bumetanide (0.5 mm), as well as flux studies with 125I- or 36C1-, indicated that this current represented Cl- secretion. The late increase in I sc appeared with a lag of 2–5 min and was maximal after 15–25 min. It corresponded to an increase in Na+ reabsorption, since it was amiloride sensitive. Bidirectional 36C1- flux measurements in aldosterone-treated monolayers maintained under open-circuit conditions showed that the large vasotocin-induced increase in Cl- permeability led, in these conditions, to a threefold increase of a baseline Cl- reabsorption. This study shows that vasotocin induces in A6-C1 cells both a rapid increase in Cl- permeability and a slower increase in Na+ transport. The Cl- permeability, which leads to Cl- secretion under short-circuit conditions, contributes, under the more physiological open-circuit conditions, to the transport of Na+ by allowing its co-reabsorption with Cl-.
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I wish to thank Uschi Bolliger for her skillful technical assistance, J.D. Horisberger for helpful discussions and suggestions and for reading the manuscript, J. Beron, J. Biber, and I. Forster for reading the manuscript, and Christian Gasser for the art work. This work was supported by Grant 31.30132.90 from the Swiss National Science Foundation.
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Verrey, F. Antidiuretic hormone action in A6 cells: Effect on apical Cl and Na conductances and synergism with aldosterone for NaCl reabsorption. J. Membarin Biol. 138, 65–76 (1994). https://doi.org/10.1007/BF00211070
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DOI: https://doi.org/10.1007/BF00211070