Summary
Pseudomonas fluorescens strain 2-79 (NRRL B-15 132) is a biocontrol agent of take-all of wheat caused by the fungus Gaeumannomyces graminis var. tritici. Strain 2-79 produces the antibiotic phenazine 1-carboxylic acid, which acts as the primary mechanism of disease suppression. As a first step toward designing efficient methods of mass producing and formulating this biocontrol agent, the regulation of growth and antibiotic production by growth factors (including purines, pyrimidines, vitamins) and minerals (supplying B3+, Ca2+, Co2+, Cu2+, Fe2+, Mg2+, Mn2+, Mo6+, Zn2+) was examined in defined liquid culture. Additions of boric acid and sulfates of iron and magnesium enhanced both cell and associated antibiotic accumulation. However, accumulation of the antibiotic alone improved with additions of zinc sulfate, ammonium molybdate, and cytosine, but worsened with addition of adenine. Interactive effects involving the sulfates of iron, magnesium, and zinc were observed, and optimal iron-magnesium and iron-zinc combinations were demonstrated with respect to biomass and antibiotic accumulation, respectively.
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Brisbane PG, Janik LJ, Tate ME, Warren RO (1987) Revised structure for the phenazine antibiotic from Pseudomonas fluorescens 2–79 (NRRL B-15 132). Antimicrob Agents Chemother 31:1967–1971
Bull CT, Weller DM, Thomashow LS (1991) Relationship between root colonization and suppression of Gaeumannomyces graminis var. tritici by Pseudomonas fluorescens strain 2–79. Phytopathology 81:954–959
Chang PC, Blackwood AC (1969) Simultaneous production of three phenazine pigments by Pseudomonas aeruginosa Mac 436. Can J Microbiol 15:439–444
Cook RJ, Veseth RJ (1991) Wheat health management. APS Press, St. Paul, Minn.
Gurusiddaiah S, Weller DM, Sarkar A, Cook RJ (1986) Characterization of an antibiotic produced by a strain of Pseudomonas fluorescens inhibitory to Gaeumannomyces graminis var. tritici and Pythium spp. Antimicrob Agents Chemother 29:488–495
Palleroni NJ (1985) Biology of Pseudomonas and Xanthomonas. In: Demain AL, Solomon NA (eds) Biology of industrial microorganisms. Benjamin/Cummings Publishing Co., Redwood City, California, pp 27–56
Thomashow LS, Weller DM (1988) Role of a phenazine antibiotic from Pseudomonas fluorescens in biological control of Gaeumannomyces graminis var. tritici. J Bacteriol 170:3499–3508
Thomashow LS, Weller DM, Bonsall RF, Pierson LS (1990) Production of the antibiotic phenazine 1-carboxylic acid by fluorescent Pseudomonas species in the rhizosphere of wheat. Appl Environ Microbiol 56:908–912
Vidaver AK (1967) Synthetic and complex media for the rapid detection of fluorescence of phytopathogenic pseudomonads: effect of the carbon source. Appl Microbiol 15:1523–1524
Webb SR (1971) Small incomplete factorial experiment designs for two- and three-level factors. Technometrics 13:243–256
Weinberg ED (ed) (1977) Microorganisms and minerals. (Microbiology series, vol 3) Marcel Dekker, New York
Weinberg ED (1986) Regulation of secondary metabolism by trace metals. In: Subramanian TAV (ed) Cell metabolism: growth and environment, vol 1. CRC Press, Boca Raton, Fla., pp 141–150
Weller DM (1988) Biological control of soilborne pathogens in the rhizosphere with bacteria. Annu Rev Phytopathol 26:379–407
Weller DM, Cook RJ (1983) Suppression of take-all of wheat by seed treatments with fluorescent pseudomonads. Phytopathology 73:463–469
Weller DM, Cook RJ (1984) Method for screening bacteria and application thereof for field control of diseases caused by Gaeumannomyces graminis. US patent no. 4456684
Zabriskie DW, Armiger WB, Phillips DH, Albano PA (1980) Trader's guide to fermentation media formulation. Trader's Protein, Memphis, Tenn., p 58
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The mention of firm names or trade products does not imply that they are endorsed or recommended by the U. S. Department of Agriculture over other firms or similar products not mentioned
Correspondence to: P. J. Slininger
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Slininger, P.J., Jackson, M.A. Nutritional factors regulating growth and accumulation of phenazine 1-carboxylic acid by Pseudomonas fluorescens 2-79. Appl Microbiol Biotechnol 37, 388–392 (1992). https://doi.org/10.1007/BF00210998
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DOI: https://doi.org/10.1007/BF00210998