Abstract
Endogenous fusicoccin (FC) or related substances were sought in horseradish (Armoracia rusticana P.) roots. An actively growing root culture was derived from plants transformed with Agrobacterium rhizogenes. The presence of FC-like substances in ethanolic extracts from roots was established in a radioreceptor binding assay with plasmalemmal FC receptors and in radioimmune analysis with an antiserum specific for FC A. FC-like ligands were found in the tissue and medium of aseptically grown culture.
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Abbreviations
- FC:
-
fusicoccin
- GC/MS:
-
gas chromatography/mass spectrometry
- RIA:
-
radioimmunoassay
- RRA:
-
radioreceptor analysis
- BSA:
-
bovine serum albumin
- Mes:
-
4-morpholineethanesulfonic acid
- HPLC:
-
high performance liquid chromatography
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Babakov, A.V., Bartova, L.M., Dridze, I.L. et al. Culture of transformed horseradish roots as a source of fusicoccin-like ligands. J Plant Growth Regul 14, 163–167 (1995). https://doi.org/10.1007/BF00210919
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DOI: https://doi.org/10.1007/BF00210919