Abstract
The biosynthesis of brassinolide (BL) in crown gall and nontransformed cells of Catharanthus roseus in which BL, castasterone (CS), typhasterol (TY), and teasterone (TE) are endogenous was investigated using deuterated TY and TE as substrates. The metabolites were analyzed by gas chromatography-mass spectrometry (GC-MS) and/or GC-selected ion monitoring (SIM). It was found that these cells converted TY to CS and BL, as well as TE to TY and CS. Because the pathway from CS to BL in the cells has already been confirmed, a biosynthetic sequence of TE → TY → CS → BL was established. Reversible conversion between TE and TY was observed.
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Biosynthesis of brassinosteroids in Catharanthus roseus. Part III. Part II of this series: Suzuki et al. (1993).
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Suzuki, H., Fujioka, S., Takatsuto, S. et al. Biosynthesis of brassinolide from teasterone via typhasterol and castasterone in cultured cells of Catharanthus roseus . J Plant Growth Regul 13, 21–26 (1994). https://doi.org/10.1007/BF00210703
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DOI: https://doi.org/10.1007/BF00210703