Summary
The conversion of glycerol to 1,3-propanediol (PD) by Clostridium butyricum DSM 5431 was studied in anaerobic culture. Growth and product formation were optimal at pH = 7.0 and T = 35° C, while aeration rate and stirrer speed were found to have no significant influence. As increasing amounts of initial glycerol led to inhibition of growth, cultivations were done in fed-batch operation. Comparative cultivations were carried out in an air-lift (ALR) and a stirred-tank reactor (STR) having equal working volumes (V L = 30 l) and no difference in product formation was found. The process was scaled up to reactor sizes of 1.2 m3 (ALR) and 2.0 m3 (STR). The same results were obtained irrespective of reactor volume as well as reactor type (STR/ALR). PD concentrations of approximately 50–58 g·l−1 and overall productivities of 2.3–2.9 g·l−1 ·h−1 could be reached.
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Biebl H (1991) Use of pH auxostat for the measurement of product inhibition. Appl Microbiol Biotechnol, in press
Forage RG, Foster MA (1982) Glycerol fermentation in Klebsiella pneumoniae: functions of the coenzyme B12 dependent glycerol and diol dehydratases. J Bacteriol 149:413
Forsberg CW (1987) Production of 1,3-propanediol from glycerol by Clostridium butyricum and other Clostridium species. Appl Environ Microbiol 53:639
Gottschalk G, Averhoff B (1990) Process for the microbial preparation of 1,3-propanediol from glycerol. European patent no. EP 0 373 230 A1
Homann T, Tag C, Biebl W-D, Schink B (1990) Fermentation of glycerol to 1,3-propanediol by Klebsiella and Citrobacter. Appl Microbiol Biotechnol 33:121
Kretschmann J, Carduck F-J, Deckwer W-D, Tag C, Biebl H (1990) Fermentative Herstellung von 1,3-Propandiol. European patent no. EP 0 361 082 A2
Nakas JP, Schaedle M, Parkinson CM, Coonley CE, Tanenbaum SW (1983) System development for linked-fermentation products of solvents from algae biomass. Appl Environ Microbiol 46:1017
Ruch FE, Lin EEC (1975) Independent constitutive expression of the aerobic and anaerobic pathways of glycerol metabolism in Klebsiella aerogenes. J Bacteriol 124:348
Schütz H, Radler F (1984) Anaerobic reduction of glycerol to propanediol-1,3 by Lactobacillus brevis and Lactobacillus buchneri. Syst Appl Microbiol 5:169
Streekstra H, Teixeira de Mattos MJ, Neijssel OM, Tempest DW (1987) Overflow metabolism during anaerobic growth of Klebsiella aerogenes NCTC 418 on glycerol and dihydroxyacetone in chemostat culture. Arch Microbiol 147:268–275
Tag C (1990) Mikrobielle Herstellung von 1,3-Propandiol. PhD Dissertation, University of Oldenburg, FRG
Tran Dinh K, Hill FF (1989) Verfahren zur Herstellung von 1,3-Propandiol. German patent no. DE 3734764 A1
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Günzel, B., Yonsel, S. & Deckwer, WD. Fermentative production of 1,3-propanediol from glycerol by Clostridium butyricum up to a scale of 2m3 . Appl Microbiol Biotechnol 36, 289–294 (1991). https://doi.org/10.1007/BF00208143
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DOI: https://doi.org/10.1007/BF00208143