Summary
The ability of Kupffer cells, spleen macrophages, pulmonary macrophages, and peritoneal macrophages (PM) to produce cytotoxic factor (CTF) was investigated in vitro. The production of CTF by Kupffer cells elicited with Corynebacterium parvum (CP) or Lactobacillus casei YIT9018 (LC9018) was higher than that of spleen, pulmonary macrophages, or PM. In addition, oxygen radical (OR) production by Kupffer cells or PM was measured. The production of OR by Kupffer cells or PM was significantly augmented by i.v. or i.p. injection of LC9018 or CP. No significant correlation was observed between the increase in OR production by Kupffer cells or PM and CTF production by Kupffer cells or PM elicited with either organism. It was suggested that activated Kupffer cells may be one important source of CTF production in serum and that the CTF-producing macrophages may be different from the OR-producing macrophages.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Adams DO, Kao KJ, Farb R, Pizzo SV (1980) Effector mechanisms of cytolytically activated macrophages. II. Secretion of a cytolytic factor by activated macrophages and its relationship to secreted neutral proteases. J Immunol 124:293
Carswell EA, Old LJ, Kassel RL, Green S, Fiore N, Willamson B (1975) An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci USA 72:3666
Currie GA (1978) Activated macrophages kill tumor cells by releasing arginase. Nature 273:758
Ferluga J, Scharlemmer HV, Baptista LC, Allison AC (1978) Production of the complement cleavage product, C3a, by activated macrophages and its tumorolytic effects. Clin Exp Immunol 31:512
Green S, Dobrjansky A, Chiasson MA (1982) Murine tumor necrosis-inducing factor: Purification effects on myelomonocytic leukemia cells. J Natl Cancer Inst 68:997
Haranaka K, Satomi N (1981) Cytotoxic activity of tumor necrosis factor (TNF) on the human cancer cells in vitro. Jpn J Exp Med 51:191
Haranaka K, Satomi N, Sakurai A (1984) Antitumor activity of murine tumor necrosis factor (TNF) against transplanted murine tumors and heterotransplanted human tumors in nude mice. Int J Cancer 34:263
Hashimoto S, Nomoto K, Matsuzaki T, Yokokura T, Mutai M (1984) Oxygen radical production by peritoneal macrophages and Kupffer cells elicited with Lactobacillus casei. Infect Immun 44:61
Helson L, Green S, Carswell EA, Old LJ (1975) Effect of tumor necrosis factor on cultured human melanoma cells. Nature 258:731
Kato I., Yokokura T., Mutai M (1983) Macrophage activation by Lactobacillus casei in mice. Microbiol Immunol 27:611
Keller R (1980) Regulatory capacities of mononuclear phagocytes with particular reference to natural immunity against tumors. In: Herberman RB (ed) Natural cell-mediated immunity against tumors. Academic Press, New York, p 1219
Kuli FC, Cuatrecasas P (1981) Possible requirement of internalization in the mechanism of in vitro cytotoxicity in tumor necrosis serum. Cancer Res 41:4885
Kuli FC, Cuatrecasas P (1981) Preliminary characterization of the tumor cell cytotoxin in tumor necrosis serum. J Immunol 126:1279
Mannel DN, Moore RN Mergenhagen SE (1980) Macrophages as a source of tumoricidal (tumor-necrotizing) factor. Infect Immun 30:523
Matthews N (1981) Tumour-necrosis factor from the rabbit, V. Synthesis in vitro by mononuclear phagocytes from various tissues of normal and BCG-injected rabbits. Br J Cancer 44:418
Nathan CF (1982) Secretion of oxygen intermediators: Role in effector function of activated macrophages. Fed Proc 41:2206
Old LJ (1976) Tumor necrosis factor. Clin Bull 6:118
Opitz HG, Neithammer D, Jackson RC, Lemke H, Huget R, Flad HD (1975) Biochemical characterization of a factor released by macrophages. Cell Immunol 18:70
Williamson BD, Carswell EA, Rubin BY, Perndergast JS, Old LJ (1983) Human tumor necrosis factor produced by human B-cell lines: Synergistic cytotoxic interaction with human interferon. Proc Natl Acad Sci USA 80:5397
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Hashimoto, S., Seyama, Y., YOkokura, T. et al. Cytotoxic factor production by kupffer cells elicited with Lactobacillus casei and Corynebacterium parvum . Cancer Immunol Immunother 20, 117–121 (1985). https://doi.org/10.1007/BF00205677
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00205677