Abstract
Polyacrylamide-gel-immobilized cells of Pseudomonas strain EPS 5028 were effective in the removal of uranium (U) from synthetic effluents. Metal accumulation was performed in an open system in columns filled with immobilized cells that were challenged with continuous flows containing U. Possible variables of the system were studied. Uranium uptake by the immobilized cells of this microorganism was affected by pH but not by temperature or flow rate. In addition, U binding could be interpreted in terms of the Freundlich adsorption isotherm indicating single-layer adsorption. The feasibility of reusing the immobilized cells was suggested after the recovery of U with a solution of 0.1 m sodium carbonate.
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Beveridge TJ, Koval SF (1981) Binding of metals to cell envelopes of Escherichia coli K-12. Appl Environ Microbiol 42:325–335
Beveridge TJ, Murray RGE (1980) Sites of metal deposition in the cell wall of Bacillus subtilis. J Bacteriol 141:876–887
Congregado F, Estañol I, Espuny MJ, Fusté MC, Manresa MA, Marquéz AM, Simón-Pujol MD (1985) Preliminary studies on the production and composition of the extracellular polysaccharide synthesized by Pseudomonas sp. EPS 5028. Biotechnol Lett 7:883–888
Fusté MC, Simón-Pujol MD, Marqués AM, Guinea J, Congregado F (1986) Isolation of a new free-living bacterium containing R-bodies. J Gen Microbiol 132:2801–2805
Gadd GM, White C, Rome L de (1988) Heavy metal and radionuclide uptake by fungi and yeasts. In: Norris PR, Kelly DP (eds) Biohydrometallurgy. Science and Technology Letters. Kew Surrey, UK, pp 421–435
Macaskie L, Dean ACR (1984) Cadmium accumulation by immobilized cells of a Citrobacter sp. Environ Technol Lett 5:177–186
Macaskie L, Dean ACR (1985) Uranium accumulation by immobilized cells of a Citrobacter sp. Biotechnol Lett 7:457–462
Macaskie L, Dean ACR (1987) Use of immobilized biofilm of Citrobacter sp. for the removal of uranium and lead from aqueous flows. Enzyme Microb Technol 9:1–4
Macaskie L, Dean ACR (1988) Uranium accumulation by immobilized biofilms of a Citrobacter sp. In: Norris PR, Kelly DP (eds) Biohydrometallurgy. Science and Technology Letters. Kew Surrey, UK, pp 556–557
Marqués AM, Bonet R, Simón-Pujol MD, Fusté MC, Congregado F (1990) Removal of uranium by an exopolysaccharide from Pseudomonas sp. Appl Microbiol Biotechnol 34:429–431
Marqués AM, Roca X, Simón-Pujol MD, Fusté MC, Congregado F (1991) Uranium accumulation by Pseudomonas sp. EPS 5028. Appl Microbiol Biotechnol 35:406–410
Nakajima A, Horikoshi T, Sakaguchi T (1982) Recovery of uranium by immobilized microorganisms. Eur J Appl Microbiol Biotechnol 16:88–91
Nasri M, Sayadi S, Barbotin JN, Dhulster P, Thomas D (1987) Influence of immobilization on the stability of pTG201 recombinant plasmid in some strain of Escherichia coli. Appl Environ Microbiol 53:740–744
Norberg AB, Persson H (1984) Accumulation of heavy metal ions by Zoogloea ramigera. Biotechnol Bioeng 26:239–246
Savvin SB (1961) Analytical use of arsenazo III. Determination of thorium, zirconium, uranium and rare earth elements. Talanta 8:673–685
Shumate II SE, Strandberg GW, Parrot JR (1978) Biological removal of metal ions from aqueous process streams. Biotechnol Bioeng Symp 8:13–20
Strandberg GW, Shumate II SE, Parrot JR (1981) Microbial cells as biosorbents for heavy metals: accumulation of uranium by Saccharomyces cerevisiae and Pseudomonas aeruginosa. Appl Environ Microbiol 41:237–245
Tobin JM, Cooper DG, Neufeld RJ (1984) Uptake of metal ions by Rhizopus arrhizus biomass. Appl Environ Microbiol 47:821–824
Tsezos M (1988) The performance of a new biological adsorbent for metal recovery. Modeling and experimental results. In: Norris PR, Kelly DP (eds) Biohydrometallurgy. Science and Technology Letters. Kew Surrey, UK, pp 465–475
Tsezos M, Volesky B (1981) Biosorption of uranium and thorium. Biotechnol Bioeng 23:583–604
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Correspondence to: M. C. Fusté
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Pons, M.P., Fusté, M.C. Uranium uptake by immobilized cells of Pseudomonas strain EPS 5028. Appl Microbiol Biotechnol 39, 661–665 (1993). https://doi.org/10.1007/BF00205071
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DOI: https://doi.org/10.1007/BF00205071