Abstract
Somatic carrot embryos were grown as batch cultures in a stirred 10-l bioreactor. Embryo production in the bioreactor was comparable to that obtained in shake-flasks. A production of about 50·103 embryos/l per day was commonly achieved with an inoculum density of 0.1% volume of tissues/volume of medium. Regularly changing of the medium increased embryo viability. A filtering unit coupled to the bioreactor was developed in order to calibrate embryos. The characteristics of the population of harvested embryos are described.
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Correspondence to: J.-P. Ducos
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Ducos, JP., Bollon, H. & Pettard, V. Production of carrot somatic embryos in a bioreactor. Appl Microbiol Biotechnol 39, 465–470 (1993). https://doi.org/10.1007/BF00205034
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DOI: https://doi.org/10.1007/BF00205034