Abstract
The effect of ethylene on in vitro plant regeneration from frond and rhizome expiants of Platycerium coronarium was investigated. Ethylene levels in the culture vessels increased with time, resulting in a decrease in the percentage of sporophytes produced. Addition of the ethylene action inhibitor silver thiosulfate resulted in an increase in the percentage of plants regenerated, indicating an inhibitory effect of ethylene on regeneration. However, the presence of 2,5-norbornadiene was not effective in reversing the effect of ethylene. Inhibitors of ethylene biosynthesis, such as cobalt chloride, salicylic acid, benzylisothiocyanate, and aminoethoxyvinylglycine, were also ineffective in increasing sporophyte regeneration. 1-Aminocyclopropane-1-carboxylic acid, the ethylene precursor, was ineffective in increasing the level of ethylene in the culture vessels. Therefore, the biosynthetic pathway of ethylene in the fern P. coronarium appears to be different from that of higher plants but similar to that of some other ferns.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
Abbreviations
- SA:
-
salicylic acid
- AVG:
-
aminoethoxyvinylglycine
- BITC:
-
benzylisothiocyanate
- STS:
-
silver thiosulfate
- ACC:
-
1-aminocyclopropane-1-carboxylic acid
References
Abeles FB, Morgan PW, Saltveit ME Jr. (1992) Ethylene in plant biology, 2nd Ed. Academic Press, San Diego
Adams DO, Yang SF (1979) Ethylene biosynthesis: Identification of 1-aminocyclopropane-1-carboxylic acid as an intermediate in the conversion of methionine to ethylene. Proc Natl Acad Sci USA 76:170–174
Avihai P, Aviv D, Galun E (1988) Ethylene and in vitro culture of potato: Suppression of ethylene generation vastly improves protoplast yield, plating efficiency and transient expression of an alien gene. Plant Cell Rep 7:403–406
Beyer EM (1976) A potent inhibitor of ethylene action in plants. Plant Physiol 58:268–271
Biddington NL (1992) The influence of ethylene in plant tissue culture. Plant Growth Regul 11:173–187
Biddington NL, Dearman AS (1986) A comparison of the effects of mechanically induced stress, ethephon and silver thiosulphate on the growth of cauliflower seedlings. Plant Growth Regul 4:33–41
Chraibi BKM, Latche A, Roustan JP, Fallot J (1991) Stimulation of shoot regeneration from cotyledons of Helianthus annuus by the ethylene inhibitors, silver and cobalt. Plant Cell Rep 10:204–207
Elmore HW, Whittier DP (1973) The role of ethylene in the induction of apogamous buds in Pteridium gametophytes. Planta 111:85–90
Higuchi H, Anaki W, Suzuki S (1987) In vitro propagation of Nephrolepis cordifolia Presl. Sci. Hort. 32:105–113
Jacobsen DW, Wang CH (1968) The biogenesis of ethylene in Penicillium digitatum. Plant Physiol 43:1959–1966
Knauss JF (1976) A partial tissue culture method for pathogenfree propagation of selected ferns from spores. Proc Fla State Hort Soc 89:363–365
Kumar PP, Reid M, Thorpe TA (1987) The role of ethylene and carbon dioxide in differentiation of shoot buds in excised cotyledons of Pinus radiata in vitro. Physiol Plant 69:244–252
LaRue TAG, Gamborg OL (1971) Ethylene production by plant cell cultures. Plant Physiol 48:394–398
Lau OL, Yang SF (1976) Inhibition of ethylene production by cobaltous ion. Plant Physiol 58:114–117
Lentini Z, Mussell H, Mutschler MA, Earle ED (1988) Ethylene generation and reversal of ethylene effects during development in vitro of rapid-cycling Brassica campestris L. Plant Sci 54:75–81
Leslie CA, Romani RJ (1986) Salicylic acid: A new inhibitor of ethylene biosynthesis. Plant Cell Rep 5:144–146
Lieberman M (1979) Biosynthesis and action of ethylene. Annu Rev Plant Physiol 30:533–591
Meijer EGM, Brown DCW (1988) Inhibition of somatic embryogenesis in tissue cultures of Medicago sativa by aminoethoxyvinylglycine, amino-oxyacetic acid, 2,4-dinitrophenol and salicylic acid at concentrations which do not inhibit ethylene biosynthesis and growth. J Exp Bot 39:263–270
Miller PM, Sweet HC, Miller JH (1970) Growth regulation by ethylene in fern gametophytes. I. Effects on protonemal and rhizoidal growth and interaction with auxin. Am J Bot 57:212–217
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 5:473–497
Osborne DJ (1989) The control role of ethylene in plant growth and development. In: Clijsters H, deProft M, Marcelle R, vanPoucke M, (eds) Biochemical and Physiological Aspects of Ethylene Production in Lower and Higher Plants. Kluwer, Dordrecht, pp 1–11
Patil SS, Tang CS (1974) Inhibition of ethylene evolution in papaya pulp tissue by benzylisothiocyanate. Plant Physiol 53:585–588
Pua EC, Chi GL (1993) De novo shoot morphogenesis and plant growth of mustard (Brassica juncea) in vitro in relation to ethylene. Physiol Plant 88:467–474
Sisler EC, Yang SF (1984) Anti-ethylene effects of cis-2-butene and cyclic olefins. Phytochemistry 23:2765–2768
Songstad DD, Duncan DR, Widholm JM (1988) Effect of 1-aminocyclopropane-1-carboxylic acid, silver nitrate and norbornadiene on plant regeneration from maize callus cultures. Plant Cell Rep 7:262–265
Tittle FL (1987) Auxin-stimulated ethylene production in fern gametophytes and sporophytes. Physiol Plant 70:499–502
Wang CH, Persyn A, Krackov J (1962) Role of the Krebs cycle in ethylene biosynthesis. Nature 195:1306–1308
Warner TR, Hickok LG (1987) (2-Chloroethyl)phosphonic acid promotes germination of immature spores of Ceratopteris richardii Brongn. Plant Physiol 83:723–725
Wee YC, Kwa SH, Loh CS (1992) Production of sporophytes from Platycerium coronarium and P. ridleyi frond strips and rhizome pieces cultured in vitro. Am Fern J 82:40–44
Yang SF, Hoffman HE (1984) Ethylene biosynthesis and its regulation in higher plants. Annu Rev Plant Physiol 35:155–189
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kwa, SH., Wee, YC. & Kumar, P.P. Role of ethylene in the production of sporophytes from Platycerium coronarium (Koenig) desv. frond and rhizome pieces cultured in Vitro . J Plant Growth Regul 14, 183–189 (1995). https://doi.org/10.1007/BF00204910
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00204910