Summary
A new lignin isolation method has been developed. Wood and pulp were subjected to ball milling, swelled in an organic solvent, and then treated with a cellulase. The enzyme digestion time could be shortened to 1 day with this method. The lignin obtained has been named Swelled Enzyme Lignin (SEL). Swelling and enzyme digestion conditions and their effects on lignins were investigated. The SEL's from wood could be directly washed with water, while those from pulp had to be washed with aqueous acetic acid because they were water soluble. The purification of crude SEL's was accomplished by extracting them with dioxane-water, and then precipitating and washing with ethyl ether. Lignin yields were 24–67% based on the total amount of lignin present. The characteristics of the SEL's were further investigated by gel-permeation chromatography (GPC), infrared and 13C nuclear magnetic resonance (NMR) spectroscopy.
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Abbreviations
- SUKP:
-
Softwood Unbleached Kraft Pulp
- BUKP:
-
Birch Unbleached Kraft Pulp
- TD:
-
Todomatsu (Abies sachalinensis) wood
- MWL:
-
Milled Wood Lignin
- MWLS (BK, TD):
-
Milled Wood Lignin of SUKP (BUKP, TD)
- SEL-C:
-
Swelled Enzyme Lignin (crude)
- SELS-C:
-
SEL-C of SUKP, other materials named in the same way
- SELS-90:
-
SEL-C of SUKP and extracted with 90% dioxane-water
- SELS-96:
-
SEL-C of SUKP and extracted with 96% dioxane-water, other materials named in the same way
References
Chang, H.-M.; Cowling, E. B.; Brown, W.; Adler, E.; Miksche, G. 1975: Comparative studies on cellulolytic enzyme lignin and milled wood lignin of sweetgum and spruce. Holzforschung. 29: 153–159
Chen, C.-L.; Robert, D. 1988: Characterization of lignin by 1H and 13C NMR spectroscopy. In: Wood, W. A.; Kellogg, S. T. (Ed.): Methods in enzymology, Vol. 161, Biomass Part B, pp. 137–174. New York: Academic Press
Glassier, W. G.; Barnett, C. A. 1979: The structure of lignin in pulps. Holzforschung. 33: 78–86
Hergert, H. L. 1971: Infrared spectra. In: Sarkanen, K. V.; Ludwig, C. H. (Ed.): Lignins, occurrence, formation, structure and reactions, pp. 268–272. New York: Wiley Interscience
Isogai, A.; Ishizu, A.; Nakano, J. 1987: Residual lignin in unbleached kraft pulp. Part I. J. Wood Chem. Technol. 7(3): 311–324
Iversen, T.; Wännström, S. 1986: Lignin-carbohydrate bonds in a residual lignin isolated from pine kraft pulp. Holzforschung. 40: 19–22
Johnson, D. B.; Moore, W. E.; Zank, L. 1961: The spectrophotometric determination of lignin in small wood samples. Tappi. 44: 793–798
Kawamura, I.; Higuchi, T. 1964: Studies on the properties of lignins of plants in various taxonomical positions II: On the I.R. Absorption spectra of lignins. J. Jap. Wood Res. Soc. 10: 200
Lin, S. Y.; Dence, C. W. (Ed.) 1992 Methods in Lignin chemistry Heidelberg: Springer, 578 pp.
Obst, J. R.; Kirk, T. K. 1988: Isolation of lignin. In: Wood, W. A.; Kellogg, S. T. (Ed.): Methods in enzymology, Vol. 161, Biomass Part B, 3–12. New York: Academic Press
van Zyl, J. D. 1978: Notes on the spectrophotometric determination of lignin in wood samples. Wood Sci. Technol. 12: 251–259
Musha, Y.; Goring, D. 1974: Klason and acid-soluble lignin content of hardwood. Wood Sci. 7: 133
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Chen, J.Y., Shimizu, Y., Takai, M. et al. A method for isolation of milled-wood lignin involving solvent swelling prior to enzyme treatment. Wood Sci.Technol. 29, 295–306 (1995). https://doi.org/10.1007/BF00202089
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DOI: https://doi.org/10.1007/BF00202089