Abstract
The patch-clamp technique was applied to vacuoles isolated from a photoautotrophic suspension cell culture of Chenopodium rubrum L. and vacuolar clamp currents, which are predominantly carried by the previously identified Ca2+-dependent slow vacuolar (SV) ion channels, were recorded. These currents, which were activated by 1-s voltage pulses of -100 mV (vacuolar interior negative) in the presence of 100 μM Ca2+ (cytosolic side), could be blocked completely and reversibly by the calmodulin antagonist W-7 [N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide] and its chlorine-deficient analogue W-5; half-maximum inhibition was found at approx. 6 μM for W-7 and 70 μM for W-5. Inhibition was reversed by addition of 1 μg · ml−1 calmodulin purified from Chenopodium cell suspensions; reversal by bovine brain calmodulin was scarcely appreciable. We conclude that cytosolic calmodulin mediates the Ca2+ dependence of the SV-channel in the Chenopodium tonoplast.
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Abbreviations
- SV-channel:
-
slowly activated, vacuolar ion channel
- W-5:
-
N-(6-aminohexyl)-1-naphthalenesulfonamide
- W-7:
-
N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide
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We acknowledge support by the Deutsche Forschungsgemeinschaft and the Bundesminister für Forschung und Technologie, Bonn, and by the Justus-Liebig-Universität Giessen (to W.B.)
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Weiser, T., Blum, W. & Bentrup, FW. Calmodulin regulates the Ca2+-dependent slow-vacuolar ion channel in the tonoplast of Chenopodium rubrum suspension cells. Planta 185, 440–442 (1991). https://doi.org/10.1007/BF00201069
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DOI: https://doi.org/10.1007/BF00201069