Abstract
Through a 5-d exposure at 14° C/8° C (day/ night), soybean (Glycine max [L.] Merr.) was acclimated to a lower temperature of 8° C. In order to assess changes in protein synthesis related to chilling acclimation, proteins were labeled in vivo with [35S]methionine, separated by two-dimensional gel electrophoresis, and the derived autoradiograms were subjected to computer analysis. Two sets of chilling-acclimation-related proteins were characterized following exposure and labeling at 8° C. One set corresponded to proteins whose synthesis was stimulated in acclimated plants in comparison with non-acclimated plants after transfer to 8° C for 2 d. The other set also displayed an enhanced synthesis in the acclimated plants versus the non-acclimated plants but after 7 d of exposure at 8° C. Most of these chilling-acclimation-related proteins were not increased during the acclimation period at 14° C. Using microsequence analysis, one of these proteins was shown to have a high sequence homology with members of the heat-shock protein (HSP 70) family.
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Abbreviations
- HSP 70:
-
heat-shock protein of 70 kDa
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Thanks are due to G. Borderies (Centre de Biologie et Physiologie Végétale, Toulouse, France) for HPLC analysis and to M. Campbell (Centre de Biologie et Physiologie Végétale, Toulouse, France) for critically reading the manuscript. This research is supported by ASEDIS-SO and CNRS (BDI cofinancée CNRS-entreprise).
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Cabané, M., Calvet, P., Vincens, P. et al. Characterization of chilling-acclimation-related proteins in soybean and identification of one as a member of the heat shock protein (HSP 70) family. Planta 190, 346–353 (1993). https://doi.org/10.1007/BF00196963
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DOI: https://doi.org/10.1007/BF00196963