Summary
This study examined the relationship between the number of nuclei in an osteoclast and its resorptive efficiency, as demonstrated by the size of the pit it can make in a mineralized tissue in 24 h in vitro. Osteoclasts released mechanically from prehatch chick long bones were cultured on dentine slices or on plastic dishes for periods of 6 or 24 h. The frequency distribution of the multinucleate tartrate-resistant acid phosphatase (TRAP)-positive cells with different numbers of nuclei was determined: the mean number of nuclei per cell was 6.92, with a mode of 4. 47% had 5 or fewer nuclei and only 11% more than 10 nuclei. The pits associated with 292 osteoclasts with known numbers of nuclei were measured using a confocal laser light microscope (Lasertec) and dedicated image analysis system, and depths, plan areas and volumes determined. There was a positive correlation between the number of nuclei per osteoclast and the volume of the pit made, but a trend for the volume resorbed per nucleus to decrease with increase in the number of nuclei per osteoclast.
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References
Addison WC (1978) The distribution of nuclei in human odontoc-lasts in whole cell preparations. Arch Oral Biol 23:1167–1171
Addision WC (1980) The effect of parathyroid hormone on the numbers of nuclei in feline osteoclasts in vivo. J Anat 130:479–486
Amano S, Hanazawa S, Kawata Y, Ohta K, Kitami H, Kitano S (1992) An assay system utilizing devitalized bone for assessment of differentiation of osteoclast precursors. J Bone Min Res 7:321–328
Athanasou NA, Puddle B, Quinn J, Woods CG (1991) Use of monoclonal antibodies to recognise osteoclasts in routinely processed bone biopsy specimens. J Clin Pathol 44:664–666
Baron R, Neff L, Louvard D. Courtoy PJ (1985) Cell-mediated extracellular acidification and bone resorption: evidence for a low pH in resorbing lacunae and localization of a 100kD lyso-somal membrane protein at the osteoclast ruffled border. J Cell Biol 101:2210–2222
Basle MF, Mazaud P, Malkani K, Chretien MF, Moreau MF, Rebel A (1988) Isolation of osteoclasts from Pagetic bone tissue. Morphometry and cytochemistry on isolated cells. Bone 9:1–6
Boyde A, Jones SJ (1979) Estimation of the size of resorption lacunae in mammalian calcified tissues using SEM stereophoto-grammetry. Scanning Electron Microsc/1979/2:393–402
Boyde A, Jones SJ (1987) Early scanning electron microscopic studies of hard tissue resorption: their relation to current concepts reviewed. Scanning Microsc 1:369–381
Boyde A, Jones SJ (1991) Pitfalls in pit measurement. Calcif Tissue Int 49:65–70
Boyde A, Jones SJ (1992) Really close up! Surveying surfaces at submicrometre resolution: the measurement of osteoclastic resorption lacunae. Photogramm Rec 14:59–84
Boyde A, Ali NN, Jones SJ (1983) Computer-aided measurement of resorptive activity of isolated osteoclasts. Proc Microsc Soc 18:357
Delaisse J-M, Boyde A, Maconnachie E, Ali NN, Sear CHJ, Eeck-hout Y, Vaes G, Jones SJ (1987) The effect of inhibitors of cysteine-proteinases and collagenase on the resorptive activity of isolated osteoclasts. Bone 8:303–313
Groessner-Schreiber B, Krukowski M, Hertweck D, Osdoby P (1991) Osteoclast formation is related to bone matrix age. Calcif Tissue Int 48:335–340
Hattersley G, Chambers TJ (1989) Generation of osteoclastic function in mouse bone marrow cultures: multinuclearity and tar-trate-resistant acid phosphatase are unreliable markers for osteoclastic differentiation. Endocrinology 124:1689–1696
Hefley TJ, Stern PH (1982) Isolation of osteoclasts from fetal rat long bones. Calcif Tissue Int 34:480–487
Holtrop ME, King GJ, Cox KA, Reit B (1979) Time-related changes in the ultrastructure of osteoclasts after injection of parathyroid hormone in young rats. Calcif Tissue Int 27:129–135
Jones SJ, Boyde A (1988) The resorption of dentine and cementum in vivo and in vitro. In: Davidovitch Z (ed) The biological mechanisms of tooth eruption and root resorption. EBSCO Media, Birmingham, Al 35233, pp 335–354
Jones SJ, Boyde A, Ali NN, Maconnachie E (1986) Variation in the sizes of resorption lacunae made in vitro. Scanning Electron Microsc 1986IV: 1571–1580
Kanehisa J, Heersche JN (1988) Osteoclastic bone resorption: in vitro analysis of the rate of resorption and migration of individual osteoclasts. Bone 9:73–79
Katoh Y, Tsuji H, Matsui H, Maruta K, Morita Y (1991) Effects of ethane-1-hydroxy-1, 1-diphosphonate on cell differentiation, and proteoglycan and calcium metabolism, in the proximal tibia of young rats. Bone 12:59–65
Kember NF (1960) Cell division in endochondral osteogenesis in young rats. J Cell Biol 14:357–370
Kukita A, Chenu C, McManus LM, Mundy GR, Roodman GD (1990) Atypical multinucleated cells form in long-term marrow coltures from patients with Paget's disease. J Clin Invest 85:1280–1286
Raff MC (1992) Social controls on cell survival and cell death. Nature 356:397–400
Ries WL, Gong JK (1982) A comparative study of osteoclasts: in situ versus smear specimens. Anat Rec 203:221–232
Ries WL, Gong JK, Gunsolley JC (1987) The distribution and kinetics of nuclei in rat osteoclasts. Cell Tissue Kinet 20:1–14
Shapiro F, Key LL, Anast C (1988) Variable osteoclast appearance in human infantile osteopetrosis. Calcif Tissue Int 43:67–76
Taylor ML, Maconnachie E, Frank K, Boyde A, Jones SJ (1990) The effect of fluoride on the resorption of dentine by osteoclasts in vitro. J Bone Min Res 5 [Suppl 1]:S121-S130
Umita S, Nicholson GC, Rowe DJ, Kent GN, Martin TJ (1991) Biphasic effect of calcitonin on tartrate-resistent acid phospha-tase activity in isolated rat osteoclasts. J Bone Min Res 6:591–597
Vignery A, Wang F, Qian HY, Benz EJ, Gilmore-Herbert M (1991) Detection of the Na+-K+- ATPase alpha 3-isoform in multinucleated macrophages. Am J Physiol 260: F704–709
Young RW (1962) Cell proliferation and specialization during endochondral ossification. A study of cell division in rat bones by the method of tritiated autoradiography. J Bone Joint Surg 428:824–839
Zambonin Zallone A, Teti A (1981) The osteoclasts of hen medullary bone under hypocalcaemic conditions. Anat Embryol 162:379–392
Zheng MH, Papadimitriou JM, Nicholson GC (1991) RNA synthesis in isolated rat osteoclasts: inhibitory effect of calcitonin. Bone 12:317–322
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Piper, K., Boyde, A. & Jones, S.J. The relationship between the number of nuclei of an osteoclast and its resorptive capability in vitro. Anat Embryol 186, 291–299 (1992). https://doi.org/10.1007/BF00185977
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DOI: https://doi.org/10.1007/BF00185977