Abstract
To study the effects of nitrogen dioxide (NO2) inhalation on lung lavage surfactant components as markers of an altered surfactant metabolism in type II pneumocytes, rats were exposed to atmospheres with increasing NO2 concentrations (0.8, 5.0, and 10.0 ppm) over 1 and 3 days. After exposure lung lavage was performed and surfactant components as well as lavagable cells analyzed. An increased number of total lavage cells was found with increasing concentration and duration of NO2 exposure. Cell distribution showed an elevation in the number of granulocytes and lymphocytes whereas the number of macrophages was, diminished. The amount of total lavage protein revealed an increase related to NO2 concentration and duration. Also the content of lavage phospholipid was increased, with a decreased portion of phosphatidylcholine (PC). Further analyses of PC showed a diminished composition of saturated fatty acids but an elevated content of the unsaturated portion. Functional studies on surfactant phospholipid extracts exhibited comparable values for the surface tension at equilibrium, as well as for the maximal and minimal surface tension of animals exposed to 0.8 ppm NO2 and controls. Higher NO2 concentrations (5 and 10 ppm) resulted in increased values for surface tension compared to controls. This was also observed with purified surfactant that was obtained from controls and from NO2-exposed rats. These experiments show that in vitro exposure of purified surfactant to NO2 atmospheres was more effective than exposure in vivo. When the structure of the surfactant proteins A was studied it was found not to be altered by the N02. The data clearly demonstrate that NO2 inhalation impaired function of surfactant components that may be used as markers of altered surfactant metabolism.
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References
Arner EC, Rhoades RA (1973) Long-term nitrogen dioxide exposure. Arch Environ Health 26:156–160
Bartlett GF (1959) Phosphorus assay in column chromatography. J Biol Chem 234:466–468
Bils RF, Christie BR (1980) The experimental pathology of oxidant and air pollutant inhalation. Int Rev Exp Pathol 21:195–293
Blank ML, Dalbey W, Nettesheim P, Price J, Creasia D, Snyder F (1978) Sequential changes in phospholipid composition in lung exposed to nitrogen dioxide. Am Rev Respir Dis 130:307–308
Blank ML, Dalbey W, Cress EA, Garfinkel S, Snyder F (1982) Pulmonary NO2 toxicity: phosphatidylcholine levels and incorporation of [3H]thymidine into DNA. Environ Res 27:352–360
Bradford MA (1976) A rapid and sensitive method for the quatitation of microgram quantities of protein using the principle of protein-dye binding. Anal Biochem 72:248–254
Bylin G, Lindvall T, Rehn T, Sundin B (1985) Effects of short-term exposure to ambient nitrogen dioxide concentrations results on human bronchial reactivity and lung function. Eur J Respir Dis 134:1203–1208
Chrzanowski P, Keller S, Cerreta J, Mandl I, Turino GM (1980) Elastin content of normal and emphysematous lung. Am J Med 69:351–358
Dobbs LG, Wright JR, Hawgood S, Gonzalez R, Venstrom K, Nellenbogen J (1987) Pulmonary surfactant and its components inhibit secretion of phosphatidylcholine from cultured rat alveolar type II cells. Proc Natl Acad Sci 84:1010–1014
Evans MJ, Cabral LJ, Stephens RJ, Freeman G (1973) Renewal of alveolar epithelium in the rat following exposure to NO2. Am J Pathol 70:175–198
Folch J, Lees M, Sloane-Stanely GH (1957) A simple method for the isolation and purification of total phospholipids from animal tissue. J Biol Chem 226:497–509
Glasser SW, Korfhagen TR, Weaver T, Pilot-Matias T, Fox JL, Whitsett JA (1987) cDNA and deduced amino acid sequence of human pulmonary surfactant-associated proteolipid SPL(Phe). Proc Natl Acad Sci USA 84:4007–4011
Hawgood S, Benson BJ, Hamilton RL (1985) Effects of a surfactant-associated protein and calcium ions on the structure and surface activity of lung surfactant lipids. Biochem 24:184–190
Ichinose T, Sagai M (1982) Studies on biochemical effects of NO2: changes of the rat antioxidative protective systems in rat lungs and lipid peroxidation by chronic exposure. Toxicol Appl Pharmacol 66:1–8
Jörres R, Magnussen H (1991) Effect of 0.25 ppm nitrogen dioxide on the response to methacholine in asymptotic asthmatic patients. Lung 169:77–85
Katyal SL, Estes LW, Lombardi, B (1977) Method for the isolation of surfactant from homogenates and lavages of lung of adult, newborn, and fetal rats. Lab Invest 36:585–592
Mohsenin V (1987) Airway response to nitrogen dioxide in asthmatic subjects. J Toxicol Environ Health 22:371–380
Morrow PE (1984) Toxicological data on NO x : an overview. J Toxicol Environ Health 13:205–227
Müller B (1990) Surfactant-phospholipid synthesis in diseased lungs (abstract). Am Rev Respir Dis 141(Suppl):630
Müller B, Barth P, von Wichert P (1992) Structural and functional impairment of rat surfactant protein A (SP-A) after in vivo and in vitro exposure to nitrogen dioxide. Am J Physiol 263:L177-L184
Norman V, Keith CH (1965) Nitrogen oxides in tobacco smoke. Nature 205:915–916
Postlethwait EM, Langford SD, Bidani A (1990) Reaction absorption of nitrogen dioxide by pulmonary epithelial lining fluid. J Appl Physiol 69:523–531
Saltzman BE (1954) Colorimetric microdetermination of nitrogen dioxide in the atmosphere. Anal Chem 26:1945–1955
Sandström T, Stjernberg N, Eklund A, Ledin MC, Bjermer L, Kolmodin-Hedman B, Lindström K, Rosenhall L, Ångström T (1991) Inflammatory cell response in bronchoalveolar lavage fluid after nitrogen dioxide exposure of healthy subjects: a dose-response study. Eur Respir J 3:332–339
Schlesinger RB, Driscoll KE, Vollmuth TA (1987) Effect of repeated exposures to nitrogen dioxide and sulfuric acid mist alone or in combination on mucociliary clearance from the lungs of rabbits. Environ Res 44:294–301
Schubotz R. (1976) Gaschromatographische Analyse von Fettsäuren aus biologischem Material. Diagnostik 6:199–202
Thurlbeck WM (ed) (1988) Pathology of the Lung. Thieme, Stuttgart-New York
Trzeciak HI, Kosmider S, Kryk K, Kryk A (1977) The effects of nitrogen oxides and their neutralization products with ammonia on the lung phospholipids of guinea pigs. Environ Res 14:87–91
Wright JR, Wager RE, Hawgood S, Dobbs LG, Clements JA (1987) Surfactant apoprotein Mr = 26.000−36.000 enhances uptake of liposomes by type II cells. J Biol Chem 262:2888–2894
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Müller, B., Schäfer, H., Barth, P. et al. Lung surfactant components in bronchoalveolar lavage after inhalation of NO2 as markers of altered surfactant metabolism. Lung 172, 61–72 (1994). https://doi.org/10.1007/BF00185077
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DOI: https://doi.org/10.1007/BF00185077