Summary
Growth properties of carrot hairy root cells in various bioreactors were investigated. A turbine-blade reactor and an immobilized rotating drum reactor were found to be advantageous for the hairy root culture because of a high oxygen transfer coefficient (k in L a). After 30 days of culture, 10 g/l of dry hairy root cells were obtained in both bioreactors and maximum growth rates (V m ) were found to be 0.63 and 0.61 g/l per day for the turbine-blade reactor and immobilized rotating drum reactor, respectively. Specific growth rates (μ) at various cultivation times were observed to be linearly proportional to X/k l a for both bioreactor configurations where X is the cell concentration. The estimated specific oxygen uptake rate of 0.34 mmol O2/g dry cells per hour compares fairly well with an experimental value of 0.3.
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Kondo, O., Honda, H., Taya, M. et al. Comparison of growth properties of carrot hairy root in various bioreactors. Appl Microbiol Biotechnol 32, 291–294 (1989). https://doi.org/10.1007/BF00184976
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DOI: https://doi.org/10.1007/BF00184976