Summary
Aureobasidium pullulans CBS 58475 produced β-xylanase with an activity of 5 units/ml culture filtrate. Xylose, xylan and complex substrates containing xylose served as strong inducers. Purification of the enzyme was achieved by two sets of gel permeation chromatography. The enzyme has an pH optimum at 4.25 and a temperature optimum at 60°C. At slightly acid pH and temperatures up to 60°C β-xylanase showed good stability. The analysis of cleavage products classified the β-xylanase as an endoenzyme. Together with an endopolygalacturonase, the β-xylanase enhanced the maceration of carrots compared to endopolygalacturonase alone.
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References
Aspinall GO (1980) Chemistry of cell wall polysaccharides. In: Preiss J (ed) The biochemistry of plants, vol 3. Carbohydrates: structure and function. Academic Press, New York, pp 473–500
Biely P (1985) Microbial xylanolytic systems. Trends Biotechnol 3:286–290
Biely P, Mislovicova D, Toman R (1985a) Soluble chromogenic substrates for the assay of endo-β-1.4-xylanases and endo-1.4-β-glucanase. Anal Biochem 144:142–146
Biely P, Marcovic O, Mislovicova D (1985b) Sensitive detection of endo-1.4-β-glucanases and endo-1.4-β-xylanases in gels. Anal Biochem 144:147–151
Biely P, MacKenzie CR, Puls J, Schneider H (1986) Cooperativity of esterases and xylanases in the enzymatic degradation of acetyl xylan. Bio/Technology 4:731–733
Biswas SR, Mishra AK, Nanda G (1987) Xylanases and β-xylosidases by Aspergillus ochracheus during growth on lignocelluloses. Biotechnol Bioeng 31:613–616
Cauchon N, Le Duy A (1984) Effect on dilution on carboxymethylcellulose and xylanase assay. Biotechnol Bioeng 26:988–991
Dekker RFH, Richards GN (1976) Hemicellulases: their occurrence, purification, properties and mode of action. Adv Carbohydr Chem Biochem 32:277–352
Höfelmann M, Kittsteiner-Eberle R, Schreier P (1983) Ultrathin-layer agar gels: a novel print technique for ultrathin-layer isoelectric focusing of enzymes. Anal Biochem 128:217–222
Görg A, Postel W, Westermeier R (1978) Ultrathin-layer isoelectric focusing in polyacrylamide gels on cellophane. Anal Biochem 89:60–70
Khan AW, Tremblay D, le Duy A (1986) Assay of xylanase and xylosidase activities in bacterial and fungal cultures. Enzyme Microb Technol 8:373–377
Laemmli UK (1970) Cleavage of structural protein during assembly of the head of bacteriophage T4. Nature 227:680–685
Leathers TD (1986) Color variants of Aureobasidium pullulans overproduce xylanase with extremely high specific activity. Appl Environ Microbiol 52:1026–1030
Leathers TD, Detroy RW, Bothast RJ (1986) Induction and glucose repression from a color variant strain of Aureobasidium pullulans. Biotechnol Lett 8:867–872
Lloberas J, Querol E, Bernues J (1988) Purification and characterization of an endo-β-1.3–1.4-d-glucanase activity fromBacillus licheniformis. Appl Microbiol Biotechnol 29:32–38
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin-phenol reagent. J Biol Chem 193:265–275
Morrissey JH (1981) Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity. Anal Biochem 117:307–310
Nelson N (1944) A photometric adaption of the Somogyi method for the determination of glucose. J Biol Chem 153:375–380
Pou-Llinas J, Driguez H (1987) d-Xylose as inducer of the xylan degrading enzyme system in the yeast Pullularia pullulans. Appl Microbiol Biotechnol 27:134–138
Poutanen K, Raettoe M, Puls J, Viikari L (1987) Evaluation of different microbial xylanolytic systems. J Biotechnol 6:49–60
Somogyi M (1952) Notes on sugar determination. J Biol Chem 195:19–23
Spiro RG (1966) Analysis of sugars found in glycoproteins. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol 8. Academic Press, New York, pp 3–26
Sreenath HK, Radola BJ (1986) The effect of removing cellulase(s) from a commercial pectinase and liquefaction of carrots. J Biotechnol 4:262–282
Steiner W, Lafferty RM, Gomes I, Esterbauer H (1987) Studies on a wild strain of Schizophyllum commune: cellulase and xylanase production and formation of the extracellular polysaccharide schizophyllan. Biotechnol Bioeng 30:169–178
Tan LUL, Wong KKY, Yu EKC, Saddler JN (1985) Purification and characterization of two d-xylanases from Trichoderma harzianum. Enzyme Microb Technol 7:425–430
Thomson NS (1983) Hemicellulose as biomass resource. In: Soltes EJ (ed) Wood and agricultural residues; research on use for feed, fuels and chemicals. Academic Press, New York, pp 101–119
Voragen AGJ, Schols HA, Siliha AI, Pilnik W (1986) Enzymic lysis of pectic substances in cell wall: some implications for fruit juice technology. In: Fishman ML, Jen JJ (eds) Chemistry and functions of pectins. ACS-Symposium Series 310, Washington, pp 320–247
Wong KKY, Tan LUL, Saddler JN (1988) Multiplicity of β-1.4-xylanase in microorganisms: functions and applications. Microbiol Rev 52:305–317
Yu EKC, Tan LUL, Chan MKH, Deschatelets L, Saddler JN (1987) Production of thermostable xylanase by a thermophilic fungus. Thermoascus auranticus. Enzyme Microb Technol 9:16–24
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Dobberstein, J., Emeis, C.C. β-Xylanase produced by Aureobasidium pullulans CBS 58475. Appl Microbiol Biotechnol 32, 262–268 (1989). https://doi.org/10.1007/BF00184971
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DOI: https://doi.org/10.1007/BF00184971