Summary
Favourable reaction conditions for the enzymatic production of 1-kestose by sucrose-1F-fructosyltransferase, SFT (EC 2.4.1.99) from Aspergillus phoenicis CBS 294.80 mycelium were established. The intracellular enzyme SFT works best at 60°C, exhibits a relatively high thermostability and possesses an alkaline pH optimum. An invertase also present in the mycelium of A. phoenicis possesses an acidic pH optimum. Consequently, around pH 8.0 sucrose is converted mainly to 1-kestose and nystose while fructose is only formed in relatively small amounts. Under optimal conditions (55° C, pH 8.0 and an initial sucrose concentration of 750 g 1-1) a yield of about 300 g 1-kestose per 1.01 reaction mixture could be achieved after 8 h.
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References
Bacon JSD (1954) The oligosaccharides produced by the action of yeast invertase preparations on sucrose. Biochem J 57:320–328
Bhatia IS, Satyanarayana MN, Srinivason M (1955) Transfructosidase from Agave vera cruz Mill. Biochem J 61:171–174
Dedonder R (1952) Les glycides du topinambour. Synthése de glucofructosanes in vitro par des extraits de divers organes de topinambour. Bull Soc Chim Biol 34:171–182
DSM Stamicarbon (1989) Fructosyl transferase and the preparation of fructose oligomers therewith. US patent no. 4 849 356
DSM Stamicarbon (1981) Inulinase. US patent no 4 397 949
Edelman J, Bacon JSD (1951) Transfructodation in extracts of the tubers of Helianthus tuberosus L. Biochem J 49:529–540
Edelman J, Dickerson AG (1966) The metabolism of fructose polymers in plants. Transfructosylation in tubers of Helianthus tuberosus L. Biochem J 98:787–794
Edelman J, Jefford TG (1968) The mechanism of fructosan metabolism in higher plants as exemplified in Heliantus tuberosus. New Phytol 67:517–531
Hayashi S, Nonokuchi M, Imada K, Ueno H (1990) Production of a fructosyl-transferring enzyme by Aureobasidium sp. ATCC 20 524. J Ind Microbiol 5:395–400
Hehre EJ (1951) Enzymic synthesis of polysaccharides: a biological type of polymerization. Adv Enzymol Biochem 11:297–337
Henry RJ, Darbyshire B (1980) Sucrose:sucrose fructosyltransferase and fructan:fructan fructosyltransferase from Allium cepa. Phytochemistry 19:1017–1020
Hestrin S, Geingold DS, Avigad G (1956) The mechanism of polysaccharide production from sucrose. Biochem J 64:340–351
Hirayama M, Sumi N, Hidaka H (1989) Purification and properties of a fructo-oligosaccharide producing β-fructofuranosidase from Aspergillus niger ATCC 20 611. Agric Biol Chem 53:66–673
Jacques NA (1985) Inhibition of the expression of cell-associated fructosyl transferase in Streptococcus salivarius by octyl-β-d-glucopyranoside. J Gen Microbiol 131:3243–3250
Meiji Seika Kaisha (1981) Zoetstof. Dutch patent application no 8 101 587
Meiji Seika Kaisha (1982) Kalorienarmes Süssungsmittel und Verfahren zur Herstellung von Nahrungsmitteln und Getränken mit niedrigem Kaloriengehalt unter Verwendung desselben. German patent application no 3 232 531
Meiji Seika Kaisha (1987) Process for the preparation of inulase. European patent application no 0 240 741
Muramatsu M, Kainuma S, Miwa T, Nakatani T (1988) Structures of some fructo-oligosaccharides produced from sucrose by mycelia of Aspergillus sydowi IAM 2544. Agric Biol Chem 52:1303–1304
Pazur JH (1952) Transfructosidation reactions of an enzyme of Aspergillus oryzae. J Biol Chem 199:217–225
Reese ET, Avigad G (1966) Purification of levansucrase by precipitation with levan. Biochim Biophys Acta 113:79–83
Shimura S, Itoh Y (1985) Fructose-transferring enzyme from Penicillium oxalicum and culture conditions for its production. Nippon Shokuhin Kogyo Gakkaishi 32:419–425
Straathof AJJ, Kieboom APG, Bekkum H van (1986) Invertase-catalysed fructosyl transfer in concentrated solutions of sucrose. Carbohydr Res 146:154–159
Yun JW, Jung KH, Oh JW, Lee JH (1990) Semibatch production of fructo-oligosaccharides from sucrose by immobilized cells of Aureobasidium pullulans. Appl Biochem Biotechnol 24/25: 299–308
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van Balken, J.A.M., van Dooren, T.J.G.M., van den Tweel, W.J.J. et al. Production of 1-kestose with intact mycelium of Aspergillus phoenicis containing sucrose-1F-fructosyltransferase. Appl Microbiol Biotechnol 35, 216–221 (1991). https://doi.org/10.1007/BF00184689
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DOI: https://doi.org/10.1007/BF00184689