Summary
Two methods for on-line biomass measurement were tested with success: (a) infrared (IR) estimation of cell components (glucosamine and ergosterol) and medium residues (sucrose and nitrogen) directly on solid medium; (b) the CO2 evolution rate during cultivation. These methods were very satisfactory for following biomass changes during a defined process. The IR measurements correlated well with manual methods. The correlation between CO2 and glucosamine measurements was very satisfactory with good precision. However, they did not permit comparison between processes.
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Desgranges, C., Georges, M., Vergoignan, C. et al. Biomass estimation in solid state fermentation II. On-line measurements. Appl Microbiol Biotechnol 35, 206–209 (1991). https://doi.org/10.1007/BF00184687
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DOI: https://doi.org/10.1007/BF00184687