Abstract
The effect of varying CO2 concentrations on the growth of beet and safflower hairy roots was measured for tissues cultured in nutrient mists and on solid media in chambers fed mixtures of humidified air supplemented with different CO2 concentrations. Hairy root tissue grown on solid media in air enriched with CO2 showed increased growth, as measured by dry weight increases vs air-fed controls. Growth increased with CO2 enrichment as much as 2.5 times more than the air-fed control for safflower at 1.0% CO2 and 1.4 times more than the air-fed control for beets at 1.5% CO2 over a 12-day period. Beet hairy root tissue was also cultured aeroponically in nutrient mists. Beet hairy root cultured aeroponically in nutrient mists enriched with 1.0% CO2 showed a 15% increase in biomass over a 7-day period vs tissue cultured in nutrient mists (with ambient air) or in shake flasks. The stimulation of root growth via CO2 enrichment reduced the time required for biomass accumulation.
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DiIorio, A.A., Cheetham, R.D. & Weathers, P.J. Carbon dioxide improves the growth of hairy roots cultured on solid medium and in nutrient mists. Appl Microbiol Biotechnol 37, 463–467 (1992). https://doi.org/10.1007/BF00180969
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DOI: https://doi.org/10.1007/BF00180969