Summary
A yeast potentiometric biosensor for glucose determination is described. After induction of glycolytic enzyme synthesis a cell suspension of the yeast Hansenula anomala is retained in calcium alginate gel on the surface of a glass electrode. This biosensor gives a Nernstian response in glucose concentration of 5·10−4–5·10−3 mol/l with a response time of 5 min and a life-time of at least 2 months. Mannose and fructose are the only significantly interfering substances. The biosensor was used for measurement of glucose concentration in urine with results comparable to those obtained by a photometric enzymatic method.
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Racek, J. A yeast biosensor for glucose determination. Appl Microbiol Biotechnol 34, 473–477 (1991). https://doi.org/10.1007/BF00180573
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DOI: https://doi.org/10.1007/BF00180573