Abstract
A constitutive blocked mutant (UV4) of Pseudomonas putida was grown in a 2.5-l fermentor on a mineral salts medium. Glucose was fed normally over an 18-h period. Gluconate reached about 5 gl−1 in the medium and then fell to zero as it was utilised. The maximum toluene dioxygenase specific activity (2 g g−1 h−1) was obtained over the last 6 h of the fermentation when the pH was fully controlled. In fermentations done at low dissolved O2 tension (DOT) values there was an overall reduction in the cellular enzyme level. When stored at 4°C in phosphate buffer, pH 7.0, harvested bacteria lost half their activity in about 90 h.
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Hack, C.J., Woodley, J.M., Lilly, M.D. et al. The production of Pseudomonas putida for the hydroxylation of toluene to its cis-glycol. Appl Microbiol Biotechnol 41, 495–499 (1994). https://doi.org/10.1007/BF00178478
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DOI: https://doi.org/10.1007/BF00178478