Summary
Glycerol-fermenting anaerobes were enriched with glycerol at low and high concentrations in order to obtain strains that produce 1,3-propanediol. Six isolates were selected for more detailed characterization; four of them were identified as Citrobacter freundii, one as Klebsiella oxytoca and one as K. pneumoniae. The Citrobacter strains formed 1.3-propanediol and acetate and almost no by-products, while the Klebsiella strains produced varying amounts of ethanol in addition and accordingly less 1,3-propanediol. Enterobacterial strains of the genera Enterobacter, Klebsiella, and Citrobacter from culture collections showed similar product patterns except for one group which formed limited amounts of ethanol, but no propanediol. Seven strains were grown in pH-controlled batch cultures to determine the parameters necessary to evaluate their capacity for 1,3-propanediol production. K. pneumoniae DSM 2026 exhibited the highest final concentration (61 g/l) and the best productivity (1.7 g/l h) whereas C. freundii Zu and K2 achieved only 35 g/l and 1.4 g/l h, respectively. The Citrobacter strains on the other hand gave somewhat better yields which were very close to the theoretical optimum of 65 mol %.
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Biebl H, Pfennig N (1981) Isolation of members of the family Rhodospirillaceae. In: Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG (eds) The prokaryotes. Springer, Berlin, Heidelberg, New York
Elm R, Falbe J, Hahn H-D, Gelbke H-P (1980) Propandiole. In: Bartholomé E, Biekert E, Hellmann H, Ley H, Weigert M, Weise E (eds) Ullmanns Encyklopädie der technischen Chemie, vol. 19. Verlag Chemie, Weinheim
Forsberg CW (1987) Production of 1,3-propanediol from glycerol by Clostridium acetobutylicum and other Clostridium species. Appl Environ Microbiol 53:639–643
Mickelson MN, Werkman CH (1940) The dissimilation of glycerol by coli-aerogenes intermediates. J Bacteriol 39:709–715
Nakas JP, Schaedle M, Parkinson CM, Coonley CE, Tanenbaum SW (1983) System development of linked-fermentation production of solvents from algal biomass. Appl Environ Microbiol 46:1017–1023
Pfennig N (1978) Rhodocyclus purpureus gen. nov. sp. nov., a ring-shaped, vitamin B12-requiring member of the family Rhodospirillaceae. Int J Syst Bacteriol 28:283–288
Schink B, Pfennig N (1982) Fermentation of trihydroxybenzenes by Pelobacter acidigallici gen. nov. sp. nov., a new strictly anaerobic, non-spore-forming bacterium. Arch Microbiol 133:195–201
Schink B, Stieb M (1983) Fermentative degradation of polyethylene glycol by a strictly anaerobic, Gram-negative, non-spore-forming bacterium, Pelobacter venetianus sp. nov. Appl Envirion Microbiol 45:1905–1913
Schütz H, Radler F (1984) Anaerobic reduction of glycerol to propanediol-1,3 by Lactobacillus brevis and Lactobacillus buchneri. Syst Appl Microbiol 5:169–178
Stieb M, Schink B (1984) A new 3-hydroxybutyrate fermenting anaerobe, Ilyobacter polytropus gen. nov. sp. nov., possessing various fermentation pathways. Arch Microbiol 140:139–146
Streekstra H, Teixeira de Mattos MJ, Neijssel OM, Tempest DW (1987) Overflow metabolism during anaerobic growth of Klebsiella aerogenes NCTC 418 on glycerol and dihydroxyacetone in chemostat culture. Arch Microbiol 147:268–275
Thimann KV (1963) The life of bacteria, 2nd edn. The MacMillan Company, New York
Toraya T, Kuno S, Fukui S (1980) Distribution of coenzyme B12-dependent diol dehydratase and glycerol dehydratase in selected genera of Enterobacteriaceae and Propionibacteriaceae. J Bacteriol 141:1439–1442
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Homann, T., Tag, C., Biebl, H. et al. Fermentation of glycerol to 1,3-propanediol by Klebsiella and Citrobacter strains. Appl Microbiol Biotechnol 33, 121–126 (1990). https://doi.org/10.1007/BF00176511
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DOI: https://doi.org/10.1007/BF00176511