Abstract
The dynamics of an Streptomyces coelicolor A3(2) culture in a 20-l computer-controlled batch bioreactor was investigated both experimentally and theoretically. In defined medium, depending on the initial conditions, the calculated value of some of the kinetic parameters were: maximum specific growth rate, 0.03 h−1; death rate constant, 1.4–6.3 × 10−3 h−1; observed biomass yield, 0.21 g cells g−1 glucose and the maintenance coefficient for the cells, 0.0448 g glucose g−1 cells h−1. According to both experimental observations and the Luedeking-Piret model, actinorhodin production was found to be growth-associated. This paper provides the first published quantitative information on the main kinetic parameters describing the activity of S. coelicolor in batch culture.
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Correspondence to: F. Mavituna
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Ozergin-Ulgen, K., Mavituna, F. Actinorhodin production by Streptomyces coelicolor A3(2): kinetic parameters related to growth, substrate uptake and production. Appl Microbiol Biotechnol 40, 457–462 (1993). https://doi.org/10.1007/BF00175730
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DOI: https://doi.org/10.1007/BF00175730