Abstract
The degradation of 2-chloroethanol by Pseudomonas putida US2 was investigated in batch, repeated batch and continuous cultures especially in a packed-bed fermenter with sand. The degradation of 2-chloroethanol was connected with a release of protons, which led to a decrease of the pH in the medium. Higher initial concentration than 25 mM 2-chloroethanol were not degraded completely because they entailed a decrease of the pH to 5.0, which inhibited further growth and degradation. P. putida US2 showed a typical repression of catabolites and diauxic growth with succinate as cosubstrate. The addition of succinate as a second substrate caused a decrease in degradation of 2-chloroethanol. Activated sludge added to adsorbed cultures in a continuous fermentation did not lead to a decrease in metabolic activity. After 2 weeks of continuous cultivation the specialized strain could be retained.
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Overmeyer, C., Rehm, HJ. Biodegradation of 2-chloroethanol by freely suspended and adsorbed immobilized Pseudomonas putida US2 in soil. Appl Microbiol Biotechnol 43, 143–149 (1995). https://doi.org/10.1007/BF00170636
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DOI: https://doi.org/10.1007/BF00170636