Summary
The fermentation of glycerol to 1,3-propanediol, acetate, and butyrate by Clostridium butyricum was studied with respect to growth inhibition by the accumulating products. The clostridia were grown in a pH-auxostat culture at low cell density and product concentration and near maximum growth rate. The products were then added individually to the medium in increasing concentrations and the resulting depression of growth rate was used as a quantitative estimate of product inhibition. Under these conditions growth was totally inhibited at concentrations of 60 g/l for 1,3-propanediol, 27 g/l for acetic acid and 19 g/l for butyric acid at pH 6.5. Appreciable inhibition by glycerol was found only above a concentration of 80 g/l. In a pH-auxostat without added products but with high cell density as well as in batch cultures the product proportions were different. The 1,3-propanediol concentration may approach the value of complete inhibition while the concentrations of acetic and butyric acids remained below these values by at least one order of magnitude. It was therefore concluded that 1,3-propanediol is the first range inhibitor in this fermentation.
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References
Biebl H, Pfennig N (1982) Isolation of members of the family Rhodospirillaceae. In: Starr MP, Stolp H, Trüper HG, Balows A, Schlegel HG (eds) The prokaryotes. Springer, Berlin Heidelberg New York, pp 267–273
Büttner R, Uebel B, Genz J (1986) Der substratlimitierte pH-Auxostat — Eine neue Methode zur kontinuierlichen Kultur. Acta Biotechnol 6:129–132
Costa JM, Moreira AR (1983) Growth inhibition kinetics for the acetone-butanol fermentation. In: Blanch HW, Papoutsakis ET, Stephanopoulos G (eds) Foundation of biochemical engineering, kinetics and thermodynamics in biological systems (American Chemical Society Symposium Series no. 207). Americal Chemical Society, Washington, DC, pp 501–512
Fond O, Jansen NB, Tsao GT (1985) A model of acetic acid and 2,3-butanediol inhibition of the growth and metabolism of Klebsiella oxytoca. Biotechnol Lett 7:727–732
Forsberg CW (1987) Production of 1,3-propanediol from glycerol by Clostridium acetobutyricum and other Clostridium species. Appl Environ Microbiol 53:634–639
Fraleigh SP, Bungay HR, Clesceri LS (1989) Continuous culture, feedback controls and auxostats. Trends Biotechnol 7:159–164
Fraleigh SP, Bungay HR, Clesceri LS (1990) Aerobic formation of ethanol by Saccharomyces cerevisiae in a computerized pH-auxostat. J Biotechnol 13:61–72
Günzel B (1991) Mikrobielle Herstellung von 1,3-Propandiol durch Clostridium butyricum und adsorptive Aufarbeitung von Diolen. PhD Thesis, University of Braunschweig
Larsson G, Enfors S-O (1990) The pH-stat as a tool for studying microbial dynamics in continuous fermentation. In: Christiansen C, Munack L, Villadsen J (eds) Abstracts of the 5th European Congress on Biotechnology Copenhagen, July 8–13. Munksgaard International, Copenhagen, p 386
Martin GA, Hempfling WP (1976) A method for the regulation of microbial population density during continuous culture at high growth rates. Arch Microbiol 107:41–47
Mas C de, Jansen NB, Tsao GV (1987) Production of optically active 2,3-butanediol by Bacillus polymyxa. Biotechnol Bioeng 31:366–377
Nakas JP, Schaedle M, Parkinson CM, Coonley CE, Tanenbaum SW (1983) System development of linked-fermentation production of solvents from algal biomass. Appl Environ Microbiol 46:1017–1023
Oltmann LF, Schoenmaker GS, Reijnders WN, Stouthamer AH (1978) Modification of the pH-auxostat culture method for the mass cultivation of bacteria. Biotechnol Bioeng 20:921–925
Zeng A-P, Biebl H, Deckwer W-D (1990) Effect of pH and acetic acid on growth and 2,3-butanediol production of Enterobacter aerogenes in continuous culture. Appl Microbiol Biotechnol 33:485–489
Zeng A-P, Deckwer W-D (1991) A model of multiproduct-inhibited growth of Enterobacter aerogenes in 2,3-butanediol fermentation. Appl Microbiol Biotechnol 35:1–3
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Biebl, H. Glycerol fermentation of 1,3-propanediol by Clostridium butyricum. Measurement of product inhibition by use of a pH-auxostat. Appl Microbiol Biotechnol 35, 701–705 (1991). https://doi.org/10.1007/BF00169880
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DOI: https://doi.org/10.1007/BF00169880