Summary
A cDNA clone, λLPO-1, encoding a major lignin peroxidase from the basidiomycete Bjerkandera adusta was isolated and characterized. The nucleotide sequence of λLPO-1 predicts a mature protein consisting of 349 amino acids with a molecular weight of 37,225 preceded by a signal peptide of 23 amino acid residues. We have also cloned and sequenced the gene encoding lignin peroxidase from B. adusta. Comparison of these sequences reveals a lignin peroxidase gene structure consisting of 1,116 bp of protein-encoding DNA that is interrupted by four intervening sequences. The putative eukaryotic regulatory sequence, a TATA box, is present at position — 75 relative to the translational initiation codon. Amino acid sequence homology between the coding regions of λLPO-1 and of the lignin peroxidase cDNA clone λML-1 from Phanerochaete chrysosporium is 61%.
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Kimura, Y., Asada, Y., Oka, T. et al. Molecular analysis of a Bjerkandera adusta lignin peroxidase gene. Appl Microbiol Biotechnol 35, 510–514 (1991). https://doi.org/10.1007/BF00169758
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DOI: https://doi.org/10.1007/BF00169758