Summary
The stabilization effect of different molecular weights of polyvinyl alcohol (PVA) upon solutions of commonly used analytical enzymes was studied. It was found that various PVA conditions could stabilize the activity of horseradish peroxidase and glucose oxidase but not β-galactosidase or alkaline phosphatase. It is expected that PVA can stabilize conjugate forms of glucose oxidase as well as horseradish peroxidase (as previously shown) but not the conjugate forms of β-galactosidase or alkaline phosphatase.
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References
Besman, M. and Coleman, J.E. (1985). J. Biol. Chem. 260, 11190–11193.
Blais, B.W. and Yamazaki, H. (1992). Immunol. Invest. 21, 581–588.
Boyd, S. and Yamazaki, H. (1994). Biotechn. Techn. 8, 123–128.
Shannon, L., Kay, E. and Lew, J.Y. (1966). J. Biol. Chem. 241, 2166–2172.
Tsuge, H., Natsuaki, O. and Ohashi, K. (1975). J. Biochem. 78, 835–843.
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Boyd, S., Letcher, K. & Yamazaki, H. Stabilization effect of polyvinyl alcohol on horseradish peroxidase, glucose oxidase, β-galactosidase and alkaline phosphatase. Biotechnol Tech 10, 693–698 (1996). https://doi.org/10.1007/BF00168482
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DOI: https://doi.org/10.1007/BF00168482