Summary
The abilities of the white-rot fungi Chrysosporium lignorum, Trametes versicolor, Phanerochaete chrysosporium and Stereum hirsutum to mineralize 3,4-dichloroaniline, dieldrin and phenanthrene were investigated. S. hirsutum did not mineralize any of the test compounds but the other strains partly mineralized them all to varying degrees. The relative degradation rates per unit biomass were T. versicolor > C. lignorum > P. chrysosporium. Evidence was obtained for the production of water-soluble metabolic intermediates but no attempt was made to characterize these. It was found that mineral salts-glucose medium supplemented with trace mineral nutrients, vitamins and 1.5 mm 3,4-dimethoxybenzyl alcohol (veratryl alcohol) resulted in the highest mineralization rate. At no time in these experiments was there detectable extracellular ligninase (lignin peroxidase) activity.
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Aitken MD, Venkatdri R, Irvine RL (1989) Oxidation of phenolic pollutants by a lignin degrading enzyme from the white-rot fungus Phanerochaete chrysosporium. Water Res 23:443–450
Asther M, Tonon F, Corrieu G, Odier E (1988) Strategies to enhanced ligninase production by Phanerochaete chrysosporium. FEMS Symp 43:333–347
Blondeau R (1989) Biodegradation of natural and synthetic humic acids by the white-rot fungus Phanerochaete chrysosporium. Appl Environ Microbiol 55:1282–1285
Bumpus JA (1989) Biodegradation of polycyclic aromatic hydrocarbons by Phanerochaete chrysosporium. Appl Environ Microbiol 55:154–158
Bumpus JA, Aust SD (1987a) Biodegradation of environmental pollutants by the white-rot fungus Phanerochaete chrysosporium: involvement of the lignin degrading system. BioEssays 6:116–120
Bumpus JA, Aust SD (1987b) Biodegradation of DDT [1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane] by the white rot fungus Phanerochaete chrysosporium. Appl Environ Microbiol 53:2001–2008
Bumpus JA, Tien M, Wright D, Aust SD (1985) Oxidation of persistent environmental pollutants by a white-rot fungus. Science 228:1434–1436
Chang H-M, Joyce TW, Kirk TK, Huynh VB (1985) Process of degrading chloroorganics by white-rot fungi. US patent no. 4 554 075
Eaton DC (1985) Mineralization of polychlorinated biphenyls by Phanerochaete chrysosporium: a ligninolytic fungus. Enzyme Microb Technol 8:209–212
Haemmerli SD, Leisola MSA, Sanglard D, Fiechter A (1986) Oxidation of benzo(a)pyrene by extracellular ligninase of Phanerochaete chrysosporium. J Biol Chem 261:6900–6903
Hallinger S, Ziegler W, Wallnofer PR, Engelhardt G (1988) Verhalten von 3,4-dichloranilin in wachsenden Pilzkulturen. Chemosphere 17:543–550
Hammel KE, Kalyanaraman B, Kirk TK (1986) Oxidation of polycyclic aromatic hydrocarbons and dibenzo-[p]-dioxins by Phanerochaete chrysosporium ligninase. J Biol Chem 261:16948–16952
Kirk TK, Schultz E, Connors WJ, Lorenz LF, Zeikus JG (1978) Influence of culture parameters on lignin metabolism by Phanerochaete chrysosporium. Arch Microbiol 117:277–285
Köhler A, Jager A, Willershausen H, Graf H (1988) Extracellular ligninase of Phanerochaete chrysosporium Burdsall has no role in the degradation of DDT. Appl Microbiol Biotechnol 29:618–620
Leisola MSA, Thanei-Wyss U, Fiechter A (1985) Strategies for the production of high ligninase activities by Phanerochaete chrysosporium. J Biotechnol 3:97–107
Mileski GJ, Bumpus JA, Jurek MA, Aust SD (1988) Biodegradation of pentachlorophenol by the white-rot fungus Phanerochaete chrysosporium. Appl Environ Microbiol 54:2885–2889
Morgan P, Watkinson RJ (1989) Microbiological methods for the cleanup of soil and ground water contaminated with halogenated organic compounds. FEMS Microbiol Rev 63:277–300
Sanglard D, Leisola MSA, Fiechter A (1986) Role of extracellular ligninases in biodegradation of benzo(a)pyrene by Phanerochaete chrysosporium. Enzyme Microb Technol 8:209–212
Tien M, Kirk TK (1984) Lignin-degrading enzyme from Phanerochaete chrysosporium: purification, characterization, and catalytic properties of a unique H2O2-requiring oxygenase. Proc Natl Acad Sci USA 81:2280–2284
Tonon F, Odier E (1988) Influence of veratryl alcohol and hydrogen peroxide on ligninase activity and ligninase production by Phanerochaete chrysosporium. Appl Environ Microbiol 54:466–472
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Morgan, P., Lewis, S.T. & Watkinson, R.J. Comparison of abilities of white-rot fungi to mineralize selected xenobiotic compounds. Appl Microbiol Biotechnol 34, 693–696 (1991). https://doi.org/10.1007/BF00167925
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DOI: https://doi.org/10.1007/BF00167925