Abstract
Five different compatible solutes, sucrose, trehalose, hydroxyectoine, ectoine, and glycine betaine, were investigated for their protective effect on Escherichia coli K12 and E. coli NISSLE 1917 during drying and subsequent storage. Two different drying techniques, freeze-drying and air-drying, were compared. The highest survival rate was observed when the non-reducing disaccharides sucrose (for E. coli K12) and trehalose (for E. coli NISSLE 1917) were added. The two tetrahydropyrimidines, hydroxyectoine and ectoine, gave protection to freeze-dried E. coli NISSLE 1917 whereas E. coli K12 was protected only by hydroxyectoine. Glycine betaine seemed to be harmful for both strains of E. coli with both drying techniques. Air0drying gave much better survival rates than freeze-drying. The two strains of E. coli differed in their ability to take up compatible solutes.
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Antheunisse J, Arkesteijn-Dijksman L (1979) Rate of drying and the survival of micro-organisms. Antonie van Leewenhoek 45:177–184
Arakawa T, Timasheff SN (1985) The stabilization of proteins by osmolytes. Biophys J 47:411–414
Bligh EG, Dyer WJ (1959) A rapid method of lipid extraction and purification. Can J Biochem Physiol 37:911–917
Boos W, Ehmann U, Forkl H, Klein W, Rimmle M, Postma P (1990) Trehalose transport and metabolism in Escherichia coli. J Bacteriol 172:3450–3461
Brown AD (1976) Microbial water stress. Bacteriol Rev 40:803–846
Bushby HVA, Marshall KC (1977) Some factors affecting the survival of root-nodule bacteria on desiccation. Soil Biol Biochem 9:143–147
Caesar AJ, Burr TJ (1991) Effect of conditioning, betaine, and sucrose on survival of rhizobacteria in powder formulations. Appl Environ Microbiol 57:168–172
Clegg JS, Seitz P, Seitz W, Hazlewood CF (1982) Cellular responses to extreme water loss: the water-replacement hypothesis. Cryobiology 19:306–316
Cox CS, Heckly RJ (1973) Effects of oxygen upon freeze-dried and freeze-thawed bacteria: viability and free radical studies. Can J Microbiol 19:189–194
Crowe JH, Carpenter JF, Crowe LM, Anchordoguy TJ (1990) Are freezing and dehydration similar stress vectors? A comparison of modes of interaction of stabilizing solutes with biomolecules. Cryobiology 27:219–231
Heckly RJ, Dimmick RL (1978) Protection kinetics of additives on survival of lyophilized bacteria. Cryobiology 15:654–658
Imhoff JF, Trüper HG (1977) Ectothiorhodospira halochloris sp. nov., a new extremely halophilic phototrophic bacterium containing bacteriochlorophyll b. Arch Microbiol 114:115–121
Jolivet Y, Larher F, Hamelin J (1982) Osmoregulation in halophytic higher plants: The protective effect of glycine betaine against the heat destabilization of membranes. Plant Sci Lett 25:193–201
Kosanke JW, Osburn RM, Shuppe GI, Smith RS (1992) Slow rehydration improves the recovery of dried bacterial populations. Can J Microbiol 38:520–525
Kunte HJ, Galinski EA, Trüper HG (1993) A modified FMOC-method for the detection of amino acid-type osmolytes and tetrahydropyrimidines (ectoines). J Microbiol Methods 17:129–136
Larsen PI, Sydne LK, Landfald B, Strom AR (1987) Osmoregulation in Escherichia coli by accumulation of organic osmolytes: betaines, glutamic acid, and trehalose. Arch Microbiol 147:1–7
Leach RH, Scott WJ (1959) The influence of rehydration on the viability of dried micro-organisms. J Gen Microbiol 21:295–307
Lippert K, Galinski EA (1992) Enzyme stabilization by ectoine-type compatible solutes: protection against heating, freezing and drying. Appl Microbiol Biotechnol 37:61–65
Marshall BJ, Coote GG, Scott WJ (1974) A study of factors affecting the survival of dried bacteria during storage. CSIRO Aust Div Food Res Tech Pap 39:1–29
Mary P, Ochin D, Tailliez R (1985) Rates of drying and survival of Rhizobium meliloti strains during storage at different relative humidities. Appl Environ Microbiol 50:207–211
Mountfort DO, Pybus V (1991) Regulatory influence on the production of gamma aminobutyric acid (GABA) by a marine pseudomonad. Appl Environ Microbiol 58:237–242
Mountfort DO, Pybus V (1992) Effect of pH, temperature and salinity on the production of gamma aminobutyric acid (GABA) from amines by marine bacteria. FEMS Microbiol Ecol 101:237–244
Nei T, Souzu H, Araki T (1966) Effect of residual moisture content on the survival of freeze-dried bacteria during storage under various conditions. Cryobiology 2:276–279
Redway KF, Lapage SP (1974) Effect of carbohydrates and related compounds on the long-term preservation of freeze-dried bacteria. Cryobiology 11:73–79
Rudolph AS, Crowe JH, Crowe LM (1986) Effect of three stabilizing agents — proline, betaine, and trehalose — on membrane phospholipids. Arch Biochem Biophys 245:134–143
Schwab KB, Gaff DF (1990) Influence of compatible solutes on soluble enzymes from desiccation-tolerant Sporolobus stapfianus and desiccation-sensitive Sporolobus pyramidalis. J Plant Physiol 137:208–215
Smirnoff N, Cumbes QJ (1989) Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28:1057–1060
Van Laere A (1989) Trehalose, reserve and/or stress metabolite? FEMS Microbiol Rev 63:201–210
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Louis, P., Trüper, H.G. & Galinski, E.A. Survival of Escherichia coli during drying and storage in the presence of compatible solutes. Appl Microbiol Biotechnol 41, 684–688 (1994). https://doi.org/10.1007/BF00167285
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DOI: https://doi.org/10.1007/BF00167285