Abstract
The effect of yeast extract (YE), iron (Fe) and cobalt (Co) on anaerobic bacterial granules grown in eight laboratory-scale upflow anaerobic sludge blanket reactors was investigated using a factorial design. The experiment was performed in three periods in which different chemical oxygen demand (COD) loading rates were applied to the reactors. The COD digestion rate and the specific activity of the bacteria were positively affected by supplementation of Fe and Co in the feed under a high COD loading rate. YE had a strong positive effect on the bacterial growth rate, but no significant effect on the specific activity of the bacteria. With Fe supplementation, an excellent COD digestion rate was maintained in the reactors, and addition of YE in the feed was not necessary. Granules with better settling properties were developed under a relatively low bacterial growth rate. With the increase in COD loading rate, the percentage of calcium increased rapidly in the granules from the reactors without YE supplementation. The bacteria grown under high COD loading rate and without an Fe supplement could be Fe-deficient. Methanothrix-like rod-shaped bacteria were dominant in the granules from all reactors.
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References
Andras E, Kennedy KJ, Richardson DA (1989) Test for characterizing settleabilitu of anaerobic sludge. Environ Technol Lett 10:463–470
APHA (1985) Standard method for the examination of water and wastewater, 6th edn. American Public Health Association, Washington, D.C.
Cook AH (1958) The chemistry and biology of yeasts. Academic Press, New York, p 569
Dubrourguier HC, Prensier G, Samain E, Albagnac G (1985) Granular methanogenic sludge. In: Palz W, Coobs J, Hall DO (eds) Energy from biomass. Elsevier, Amsterdam, pp 542–546
Dubourguier HC, Buisson MN, Tissier JP, Prensier G, Albagnac G (1987) Structural characteristics and metabolic activities of granular methanogenic sludge on a mixed defined substrate. In: Lettinga G, Zehnder AJB, Grotenhuis JTC, Hulshoff Pol LW (eds) Granular anaerobic sludge; microbiology and technology. Proceedings of the GASMAT workshop, 25–27 October, 1987, Lunteren, Netherlands, Pudoc, Wageningen, pp 78–86
Goodwin JAS, Wase DAJ, Foster CF (1990) Effects of nutrient limitation on the anerobic upflow sludge blanket reactor. Enzyme Microb Technol 12:877–884
Hoban DJ, Berg L van den (1979) Effect of iron on conversion of acetic acid to methane during methanogenic fermentation. J Appl Bacteriol 47:153–159
Hulshoff Pol LW, Zeeuw WJ de, Velzeboer CTM, Lettinga G (1983) Granulation in UASB-reactors. Water Sci Technol 15:291–304
Hulshoff Pol LW, Worp JJM van de, Lettinga G, Beverloo WA (1986) Physical characterization of anaerobic granular sludge. In: Proceedings of the NVA/EWPCA Conference. Anaerobic treatment. A grown-up technology, 15–19 September 1986, Aquatech '86, Amsterdam, pp 89–101
Jarrell KF, Sprott GD (1982) Nickel transport in Methanobaterium bryantii. J Bacteriol 151:1195–1203
Kenealy WR, Zeikus JG (1982) Characterization and function of phosphoenolpyruvate carboxylase in Methanobacterium thermoautotrophicum. FEMS Microbiol Lett 14:7–10
Knetchel RJ (1977) A more economical method for the determination of chemical ozygen demand. Water Waste Eng 14:25–27
Kosaric N, Blaszczyk R, Orphan L, Lynch C, Maat D (1991) Hydraulic impact on granules in UASB reactors. Water Pollut Res J Can 26:453–474
Kuhn W, Fiebeg K, Hippe H, Mah RA, Huser BA, Gottschalk G (1983) Distribution of cytochromes in methanogenic bacteria. FEMS Microbiol Lett 20:407–410
Lettinga G, Velsen AFM van, Hobma SW, Zeeuw W de, Klapwijk A (1980) Use of the upflow sludge blanket (USB) reactor concept for biological wastewater treatment, especiallu for anaerobic treatment. Biotechnol Bioeng 17:699–734
Lettinga G, Field JA, Sierra-Alvarez R, Lier JB van, Rintala J (1991) Future perspectives for the anaerobic treatment of forest industry wastewaters. Water Sci Technol 24:91–102
Mahoney EM, Varangu LK, Cairns WL, Kosaric N, Murray RGE (1987) The effect of calcium on microbial aggregation during UASB reactor start-up. Water Sci Technol 19:249–260
Oleszkiewicz JA (1989) Granulation in anaerobic sludge bed reactors treating food industry wastes. Biol Wastes 27:217–235
Scherer P, Lippert H, Wolff G (1983) Composition of the major elements and trace elements of 10 methanogenic bacteria determined by inductively coupled plasma emission spectrometry. Biol Trace Elem Res 5:149–163
Takashima M, Speece RE (1989) Mineral nutrient requirements for high-rate methane fermentation of acetate at low SRT. J Water Pollut Control Fed 61:1645–1650
Takashima M, Speece RE (1990) Mineral requirements for methane fermentation. Crit Rev Environ Control 19:465–479
Whitman WB, Ankwanda E, Wolfe RS (1982) Nutrition and carbon metabolisms in Methanosarcina voltae. J Bacteriol 149:852–863
Zeeuw W de (1987) Granular in sludge UASB reactors. In: Lettinga G, Zehnder AJB, Grotenhuis JTC, Hulshoff Pol LW (eds) Granular anaerobic sludge; microbiology and technology. Proceedings of the GASMAT workshop, 25–27 October 1987, Lunteren, Netherlands, Pudoc, Wageningen, pp 132–145
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Shen, C.F., Kosaric, N. & Blaszczyk, R. Properties of anaerobic granular sludge as affected by yeast extract, cobalt and iron supplements. Appl Microbiol Biotechnol 39, 132–137 (1993). https://doi.org/10.1007/BF00166862
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DOI: https://doi.org/10.1007/BF00166862