Abstract
A study was made to compare the production of pectinase by Aspergillus niger CH4 in solid-state (SSF) and submerged (SmF) fermentations. Production of endo- (endo-p) and exo-pectinase (exo-p) by SSF was not reduced when glucose, sucrose or galacturonic acid (up to 10%) were added to a culture medium containing pectin. Moreover, both activities increased when concentrations of the carbon sources were also increased. In SmF, these activities were strongly decreased when glucose or sucrose (3%) was added to culture medium containing pectin. The addition of galacturonic acid affected endo-p activity production to a lesser extend than exo-p. Final endo-p and exo-p activities in SSF were three and 11 times higher, respectively, than those obtained in SmF. The overall productivities of SSF were 18.8 and 4.9 times higher for endo-p and exo-p, respectively, than those in SmF. These results indicate that regulatory phenomena, such as induction-repression or activation-inhibition, related to pectinase synthesis by A. niger CH4 are different in the two types of fermentation.
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References
Aguilar G, Huitrón C (1986) Application of fedbatch cultures in the production of extracellular pectinases by Aspergillus sp. Enzyme Microb Technol 8:541–545
Aguilar G, Huitrón C (1987) Stimulation of the production of extracellular pectinolytic activities of Aspergillus sp. by galacturonic acid and glucose addition. Enzyme Microb Technol 9:690–696
Aguilar G, Huitrón C (1990) Constitutive exopectinase produced by Aspergillus sp. CH-Y-1043 on different carbon sources. Biotechnol Lett 12:655–660
Angelova MB, Bukaro IV, Grigorov NS (1987) Regulating the biosynthesis of polymethylgalacturonase from strain Aspergillus niger 26 by the mechanism of catabolic repression. C Rendus Acad Bulg Sci Biol Microbiol 40:93–95
Brawman JW (1981) Application of enzymes in fruit juice technology. In: Birch GG, Blakerbrough N, Barker JK (eds) Enzymes and food processing. Applied Science Publishers, London, pp 129–147
Fogarty WM, Kelly CT (1983) Pectic enzymes. In: Fogarty WM (ed) Microbial enzyme biotechnology. Applied Science Publishers, London, pp. 131–182
Fogarty WM, Ward OP (1974) Pectinases and pectic polysaccharides. Prog Ind Microbiol 13:59–119
Kargi F, Curme AJ (1985) Solid state fermentation of sweet sorghum to ethanol in a rotatory drum fermentor. Biotechnol Bioeng 27:1122–1125
Kerzezs I (1955) Pectic Enzymes. Methods Enzymol 1:158–166
Leone G, Heuvel Van den J (1986) Regulation of carbohydrates of the sequential in vitro production of pectic enzymes by Botrytis cinerea. Can J Bot 65:2133–2141
Maldonado M, Stresser A, Callieri D (1989) Regulatory aspects of the synthesis of polygalacturonase and pectinesterase by Aspergillus niger sp. Sci Aliments 9:101–110
Miller L (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugars. Anal Chem 31:426–428
Mitchell DA, Greenfield PF, Doelle HW (1988) Development of a solid-state fermentation model. Biotechnol Tech 2:1–6
Nisio M, Tai K, Nagai S (1979) Hydrolase production by Aspergillus niger in solid state cultivation. Eur J Appl Microbiol Biotechnol 8:263–270
Perley AF, Page OT (1971) Differential induction of pectolytic enzymes of Fusarium roseum (Lk.) emend. Snyder and Hansen. Can J Microbiol 17:415–420
Raimbault M, Alazard D (1980) Culture method to study fungal growth in solid fermentation. Eur J Appl Microbiol 9:199–209
Robbins RH (1968) Clarification of fruit juices. Process Biochem 3:38–40
Siessere V, Said S (1989) Pectic enzymes production in solid state fermentations using citrus pulp pellets by Thalaromyces flavus, Tubercularia vulgaris and Penicillium charlessi. Biotechnol Lett 11:343–344
Torakazu T, Hirokasu K, Atsuhiko S, Toshio E (1975) Inhibition of accumulation of polygalacturonase forming activity during catabolic repression in Aspergillus niger. J Ferment Technol 53:409–412
Trejo-Hernandez MR, Oriol E, Lopez-Canales A, Roussos S, Viniegra-González G, Raimbault M (1991) Production of pectinases by Aspergillus niger by solid fermentation on support. Micol Neotrop Apl 4:49–62
Zetelaki K (1976) Optimal carbon source concentrations for the pectolytic enzymes formation of aspergilli. Process Biochem 11:11–20
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Correspondence to: E. Favela-Torres
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Solís-Pereira, S., Favela-Torres, E., Viniegra-González, G. et al. Effects of different carbon sources on the synthesis of pectinase by Aspergillus niger in submerged and solid state fermentations. Appl Microbiol Biotechnol 39, 36–41 (1993). https://doi.org/10.1007/BF00166845
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DOI: https://doi.org/10.1007/BF00166845