Summary
At water activities (a w) of 0.998 (no osmoticum) and 0.960 a w(NaCl), the affinity (K m) of glycerol transport by Zygosaccharomyces rouxii was 25.6 and 6.4 mmol/l respectively. The maximum uptake rate (V max) was ca. 2.3 μmol/g/min at both a w's. However, at an a wof 0.960 using polyethylene glycol (PEG) 400 the K mand V max for glycerol transport increased to 61.1 mmol/l and 32.2 μmol/g per minute respectively. This suggests that different glycerol transport mechanisms operate during stress by the two osmotica. The addition of uncouplers (2,4-dinitrophenol or carbonylcyanide-m-chlorophenylhydrazine) resulted in the outflow of accumulated [14C]glycerol from Z. rouxii after on osmotic upshock indicating that an active transport mechanism was operative. The transport mechanism was specific for glycerol since other polyols (mannitol, meso-erythritol and arabitol) had no effect on the uptake rate. During upshock from 0.998 to 0.960 a w(NaCl), a transient increases in the rate of [14C]glycerol uptake was observed. However, if PEG 400 was used as osmoticum, the rate of glycerol uptake failed to increase.
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References
Adler L, Blomberg A, Nilsson A (1985) Glycerol metabolism and osmoregulation in the salt-tolerant yeast Debaryomyces hansenii. J Bacteriol 162:300–306
Brown AD (1978) Compatible solutes and extreme water stress in eukaryotic micro-organisms. Adv Microb Physiol 17:181–242
Cooper TG (1982) Transport in Saccharomyces cerevisiae. In: Strathern JN, Jones EW, Broach JR (eds) The molecular biology of the yeast Saccharomyces. Metabolism and gene expression. Cold Spring Harbor Laboratory, New York
Eck JH van, Prior BA, Brandt EV (1989) Accumulation of polyhydroxy alcohols by Hansenula anomala in response to water stress. J Gen Microbiol 135:3505–3513
Edgley M, Brown AD (1978) Response of xerotolerant and non-tolerant yeasts to water stress. J Gen Microbiol 104:343–345
Gancedo G, Gancedo JM, Sols A (1968) Glycerol metabolism in yeasts. Pathways of utilization and production. Eur J Biochem 5:165–172
Höfer M (1981) Transport across biological membranes. Pitman Publishing, London, pp 36–70
Lucas C, Uden N van (1986) Transport of hemicellulose monomers in the xylose-fermenting yeast Candida shehatae. Appl Microbiol Biotechnol 23:491–495
Maxwell WA, Spoerl E (1971) Mannitol uptake by Saccharomyces cerevisiae. J Bacteriol 105:753–758
Prasad R, Rose AH (1986) Involvement of lipids in solute transport in yeasts. Yeast 2:205–220
Prior BA, Casaleggio C, Vuuren HJJ van (1977) Psychrometric determination of water activity in the high a wrange. J Food Prot 40:537–539
Reed RH, Chudek JA, Foster R, Gould C-M (1987) Osmotic significance of glycerol accumulation in exponential growing yeasts. Appl Environ Microbiol 53:2119–2123
Robinson RA, Stokes RH (1959) Electrolytic solutions, 2nd edn. Butterworth Scientific Publications, London, pp 461
Schwartz A, Nagano K, Nakao M, Lindenmayer GE, Allen JC (1971) The sodium- and potassium-activated adenosine triphosphate system. Methods Pharmacol 1:361–388
Spencer JFT, Spencer DM (1978) Production of polyhydroxy alcohols by osmotolerant yeast. In: Rose AH (ed) Primary products of metabolism, vol 2. Academic Press, London, pp 393–425
Steinkraus KH, Ayres R, Olek A, Farr D (1985) Factors influencing permeability of the cell membrane of the osmotolerant yeast Saccharomyces rouxii grown in the presence and absence of 18% NaCl:I. Na+/K+-activated Mg2+-dependent ATPase. Int J Food Microbiol 1:291–299
Tunblad-Johansson I, Adler L (1987) Effect of sodium chloride concentration on phospholipid fatty acid composition of yeasts differing in osmotolerance. FEMS Microbiol Lett 43:275–278
Uden N van (1967) Transport limited fermentation and growth of Saccharomyces cerevisiae and its competitive inhibition. Arch Microbiol 58:155–168
Watanabe Y, Takakuwa M (1984) Effect of sodium chloride on lipid composition of Saccharomyces rouxii. Agric Biol Chem 48:2415–2422
Watanabe Y, Takakuwa M (1987) Change of lipid composition of Zygosaccharomyces rouxii after transfer to high sodium chloride culture medium. J Ferment Technol 65:365–369
Wickerham LJ (1951) Taxonomy of yeasts. United States department of Agriculture, Washington DC: Technical Bulletin no. 1029
Zyl PJ van, Prior BA (1990) Water relations of polyol accumulation by Zygosaccharomyces rouxii in continuous culture. Appl Microbiol Biotechnol 33:12–17
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van Zyl, P.J., Kilian, S.G. & Prior, B.A. The role of an active transport mechanism in glycerol accumulation during osmoregulation by Zygosaccharomyces rouxii . Appl Microbiol Biotechnol 34, 231–235 (1990). https://doi.org/10.1007/BF00166787
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DOI: https://doi.org/10.1007/BF00166787