Abstract
When an initial cell loading of about 30–40 µg chlorophyll (Chl)·g−1 gel and alginate suspension of 3% (w/v) were used for immobilization of Chlamydomonas reinhardtii, the resulting cell beads showed optimum nitrite uptake rate, at 30° C and pH 7.5, of 14 µmol NO sup−inf2 ·mg−1 Chl·h−1, the photosynthetic and respiratory activities being about 120 µmol O2 produced·mg−1 Chl·h−1, and 40 µmol O2 consumed ·mg−1 Chl·h−1, respectively. The nitrite uptake activity required CO2 in the culture and persisted after 8 days of cells immobilization, or in the presence of 0.2 mm ammonium in the medium. Our data indicate that alginate-entrapped C, reinhardtii cells may provide a stable and functional system for removing nitrogenous contaminants from waste-waters.
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Vílchez, C., Vega, J.M. Nitrite uptake by Chlamydomonas reinhardtii cells immobilized in calcium alginate. Appl Microbiol Biotechnol 41, 137–141 (1994). https://doi.org/10.1007/BF00166096
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DOI: https://doi.org/10.1007/BF00166096