Summary
The effects of various iron-complexing substances on the growth of Corynebacterium glutamicum in synthetic medium were investigated. The data obtained indicate that C. glutamicum has an absolute requirement for the presence of an iron-complexing compound as a growth factor for rapid and abundabnt growth in synthetic media. This requirement can be met by adding low concentrations (10−5 M) of certain dihydroxyphenols (catechol, protocatechuate) or relatively high concentratuions (0.1%) of citrate to the medium or by autoclaving a small amount of glucose together with other media components. The addition of catechol or protocatechuate to synthetic media has advantages over media preparation with citrate or autoclaved glucose. In the described synthetic broth supplemented with catechol or protocatechuate growth is largely independent of the iron content of the medium in a range between 0.037 and 0.37 mM.
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References
Griffiths E (1987) Iron in biological systems. In: Bullen JJ, Griffiths E (eds) Iron and infection. Wiley, New York, pp 1–25
Guirard BM (1958) Microbial nutrition. Ann Rev Microbiol 12:247–278
Hall RM, Ratledge C (1986) Distribution and application of mycobactin for the characterization of species within the genus Rhodococcus. J Gen Microbiol 132:853–856
Katsumata R, Ozaki A, Oka T, Furuya A (1984) Protoplast transformation of glutamate-producing bacteria with plasmid DNA. J Bacteriol 159:306–311
Lankford CE (1973) Bacterial assimilation of iron. CRC Crit Rev Microbiol 2:273–331
Lankford CE, Kustoff TY, Sergeant TP (1957) Chelating agents in growth initiation of Bacillus globigii. J Bacteriol 74:737–748
Macham LP, Ratledge C (1975) A new group of water-soluble iron-binding compounds from mycobacteria: the exochelins. J Gen Microbiol 89:379–382
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Martin JF, Santamaria R, Sandoval H, Real G del, Mateos LM, Gil JA, Aguilar A (1987) Cloning systems in amino acid-producing corynebacteria. Bio/Technology 5:137–146
Nakayama K, Sato Z, Kinoshita S (1964a) Growth of a glutamic acid producing bacterium and related bacteria. I. Effect of iron salts, ferrichrome, amino acids and some other compounds. J Gen Appl Microbiol 10:143–155
Nakayama K, Sato Z, Tanaka H, Kinoshita S (1964b) Growth of a glutamic acid producing bacterium and related bacteria. II. Effect of chelating agent and its relation to inorganic salt. J Gen Appl Microbiol 10:181–199
Osten CH von der, Gioannetti C, Sinskey AJ (1989) Design of a defined medium for growth of Corynebacterium glutamicum in which citrate facilitates iron uptake. Biotechnol Lett 11:11–16
Peoples OP, Liebl W, Bodis M, Maeng PJ, Follettie MT, Archer JA, Sinskey AJ (1988) Nucleotide sequence and fine structural analysis of the Corynebacterium glutamicum hom-thrB operon. Mol Microbiol 2:63–72
Peters WJ, Warren RAJ (1968) Phenolic acids and iron transport in Bacillus subtilis. Biochim Biophys Acta 165:225–232
Ratledge C, Chaudhry MA (1971) Accumulation of iron-binding phenolic acids by Actinomycetales and other organisms related to the mycobacteria. J Gen Microbiol 66:71–78
Russell LM, Cryz SJ Jr, Holmes RK (1984) Genetic and biochemical evidence for a siderophore-dependent iron transport system in Corynebacterium diphtheriae. Infect Immun 45:143–149
Salton MR (1964) Requirement of dihydroxyphenols for the growth of Micrococcus lysodeikticus in synthetic media. Biochim Biophys Acta 86:421–422
Santamaria R, Gil JA, Mesas JM, Martin JF (1984) Characterization of an endogenous plasmid and development of cloning vectors and a transformation system in Brevibacterium lactofermentum. J Gen Microbiol 130:2237–2246
Sergeant TP, Lankford CE, Traxler RW (1957) Initiation of growth of Bacillus species in a chemically defined medium. J Bacteriol 74:728–736
Snow GA, White AJ (1969) Chemical and biological properties of mycobactins isolated from various mycobacteria. Biochem J 115:1031–1037
Spiro TG, Bates G, Saltman P (1967) The hydrolytic polymerization of ferric citrate. II. The influence of excess citrate. J Amer Chem Soc 89:5559–5562
Walsh BL, O'Dor J, Warren RAJ (1971) Chelating agents and the growth of Micrococcus lysodeikticus. Can J Microbiol 17:593–597
Yoshihama M, Higashiro K, Rao EA, Akedo M, Shanabruch WG, Follettie MT, Walker GC, Sinskey AJ (1985) Cloning vector system for Corynebacterium glutamicum. J Bacteriol 162:591–597
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Liebl, W., Klamer, R. & Schleifer, KH. Requirement of chelating compounds for the growth of Corynebacterium glutamicum in synthetic media. Appl Microbiol Biotechnol 32, 205–210 (1989). https://doi.org/10.1007/BF00165889
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DOI: https://doi.org/10.1007/BF00165889