Summary
For the production of a herbicide, 5-amino-levulinic acid (ALA), from anaerobic digestion liquor, the utilization of the photosynthetic bacterium, Rhodobacter sphaeroides was examined. This bacterium could produce ALA extracelularly from this liquor with the addition of levulinic acid (LA), an inhibitor of ALA dehydratase (ALAD), and glycine, a precursor of ALA biosynthesis in the Shemin pathway. Succinate (another precursor) addition was unnecessary for ALA production. When repeated additions of LA were made together with glycine ALA production was significantly enhanced. However, above three additions of LA, ALA production was not further enhanced. The maximum value of ALA production attained was 4.2 mM (0.63 g/ 1), which was over double that of other ALA producers such as Chlorella vulgaris. Propionic acid was predominantly utilized compared with other lower fatty acids, suggesting that this might be converted to ALA via succinyl-coenzyme A (CoA) in the methylmalonyl-CoA pathway.
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References
Aubart C (1983) Anaerobic digestion of pig and cattle manure in large scale digestors and power production from biogas. Energy Biomass Waste 7:741–766
Beale SI (1970) The biosynthesis of δ-aminolevulinic acid in Chlorella. Plant Physiol 45:504–506
Cooney CL, Wise DL (1975) Thermophilic anaerobic digestion of solid waste for fuel gas production. Biotechnol Bioeng 17:1119–1135
Fisher JR (1981) The engineering, economics, and management of a swine manure digestor. Agric Energy 2:379–384
Hobson PN, Shaw BG (1973) The anaerobic digestion of waste from an intensive pig unit. Water Res 7:437–449
Inno Y, Kameoka T, Sakimoto M, Abe K (1987) Biomass production and the removal of nutritional elements from the purified water of swine waste. Mizushyorigijutsu (Jpn) 28:83–89
Kobayshi M, Kurata S (1978) Mass culture and cell utilization of photosynthetic bacteria. Process Biochem 13:27–30
Lascelles J (1978) Regulation of pyrrol synthesis. In: Clayton RK, Sistrom WR (eds) The photosynthetic bacteria Plenum Press, New York, pp 795–808
Maruyama K (1979) The metabolic pathway of propionate in Rhodopseudomonas sphaeroides. S Agric Biol Chem 43:2385–2386
Maruyama K, Kitamura H (1975) Some effects of propionate on the growth of Rhodopseudomonas sphaeroides S. Agric Biol Chem 39:1521–1524
Rebeiz CA, Montazer-Zouhoor A, Hoppen H, Wu SM (1984) Photodynamic herbicides: 1. Concept and phenomenology. Enzyme Microb Technol 6:390–401
Sasaki K, Hayashi M, Nagai S (1978) Growth on acetate media of Rhodopseudomonas sphaeroides S and its vitamin B12 biosynthesis. J Ferment Technol 56:200–206
Sasaki K, Ikeda S, Nishizawa Y, Hayashi M (1987a) Production of 5-aminolevulinic acid by photosynthetic bacteria. J Ferment Technol 65:511–515
Sasaki K, Kidani T, Emoto Y, Hamaoka T (1987b) Experimental methane production from swine waste and the performance of biogas engine. J Soc Agric Struct (Jpn) 18:38–46
Sasaki K, Noparatnaraporn N, Nishizawa Y, Hayashi M, Nagai S (1988) Production of herbicide, 5-aminoevulinic acid by a photosynthetic bacterium, Rhodobacter spharoides. Annual Reports of International Center of Cooperative Research in Biotechnology (Osaka University, Japan) 11:375–377
Vrati S (1984) Single cell protein production by photosynthetic bacteria grown on the clarified effluents of biogas plant. Appl Microbiol Biotechnol 19:199–202
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Sasaki, K., Tanaka, T., Nishizawa, Y. et al. Production of a herbicide, 5-aminolevulinic acid, by Rhodobacter sphaeroides using the effluent of swine waste from an anaerobic digestor. Appl Microbiol Biotechnol 32, 727–731 (1990). https://doi.org/10.1007/BF00164749
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DOI: https://doi.org/10.1007/BF00164749