Summary
Fermentation yields of Lactobacillus plantarum were measured at controlled pH between 4.0 and 8.0 and initial lactate concentrations of 0–90 g/l. Optimal growth conditions at pH 6.0 without addition of lactate gave a growth rate of 0.57 h−1 and 20 g dry biomass/mol ATP formed (Y ATP). The pH variations resulted in a decrease in growth rate but the effect on Y ATPwas insignificant. The addition of lactate to the medium at 0 h resulted in linear decrease in the growth rate of the culture, and all the metabolic activities were completely inhibited at 110 g/l. The Y ATPand biomass/ substrate yield (Y X/S) remained fairly steady up to 33 g lactate/l, beyond which both yields decreased considerably.
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References
Bauchop T, Elsden SR (1960) The growth of micro-organisms in relation to their energy supply. J Gen Microbiol 23:457–469
Belaïch JP (1986) Le rendement de la croissance et la biomasse active dans les biotopes anaérobies. Reprod Nutr Dev 26:137–153
Bibal B, Goma G, Vayssier Y, Pareilleux A (1988) Influence of pH, lactose and lactic acid on the growth of Streptococcus cremoris: a kinetic study. Appl Microbiol Biotechnol 28:340–344
Brink BT, Konings WN (1982) Electrochemical proton gradient and lactate concentration gradient in Streptococcus cremoris cells grown in batch culture. J Bacteriol 152:682–686
Brink BT, Otto R, Hansen UP, Konings WN (1985) Energy recycling by lactate efflux in growing and non growing cells of Streptococcus cremoris. J Bacteriol 162:383–390
Kemp TL, Nazmul Karim M, Linden JC (1989) Response surface optimization of Lactobacillus plantarum batch growth. Biotechnol Lett 11:817–820
Lindgren S, Bromander A, Pettersson K (1988) Evaluation of silage additives using scale-model silos. Swed J Agric Res 18:41–49
Maloney PC, Hansen FC (1982) Stoichiometry of proton movements coupled to ATP synthesis driven by a pH gradient in Streptococcus lactis. J Membr Biol 66:63–75
Murphy MG, O'Connor L, Walsh D, Condon S (1985) Oxygen dependent lactate utilzation by Lactobacillus plantarum. Arch Microbiol 141:75–79
Otto R, Hugenholtz J, Konings WN, Veldkamp H (1980) Increase of molar growth yield of Streptococcus cremoris for lactose as a consequence of lactate consumption by Pseudomonas stutzeri in mixed culture. FEMS Microbiol Lett 9:85–88
Oxenburg MS, Snoswell AM (1965) Use of molar growth yields for the evaluation of energy-producing pathways in Lactobacillus plantarum. J Bacteriol 89:913–914
Rhee SK, Pack MY (1980) Effect of environmental pH on fermentation balance of Lactobacillus bulgaricus. J Bacteriol 144:217–221
Seale DR (1986) Bacterial inoculants as silage additives. J Appl Bacteriol Symp Suppl 61:9S–26S
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Giraud, E., Lelong, B. & Raimbault, M. Influence of pH and initial lactate concentration on the growth of Lactobacillus plantarum . Appl Microbiol Biotechnol 36, 96–99 (1991). https://doi.org/10.1007/BF00164706
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DOI: https://doi.org/10.1007/BF00164706