Summary
Cells of Saccharomyces cerevisiae (ATCC 24858) were encapsulated in the calcium alginate membrane and cultured. Swelling of the capsule was prevented by adding 0.2 g CaCl2 to 1 L growth medium. The dry cell concentration based on the inner volume of the capsule reached 309 g/L, which was much higher than could be obtained by cell entrapment. All the cells remained inside the capsule during the cultivation. The flux of CO2 through the capsule membrane increased approximately twice by adding a nonionic surfactant to the CaCl2 solution during the step of capsule formation.
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References
Chang, T. M. S. (1972) Artificial Cells, Charles C. Thomas Pub., Springfield, Illinois.
Cheetham, P. S. J., K.W. Blunt and C. Bucke (1979) Biotechnol. Bioeng., 21, 2155–2168.
Kierstan, M. and C. Bucke (1979) Biotechnol Bioeng., 19, 387–397.
Klein, J. and F. Wagner (1979) in “Characterization of Immobilized Biocatalysts”, K. Buchholz (ed.), Verlag Chemie, Germany, 265–335.
Knazek, R.A., P. M. Gullino, P. O. Kohler and R. L. Dedrick (1972) Science, 178, 65–67.
Lee, C. W. and H. N. Chang (1987) Biotechnol. Bioeng., 29, 1105–1112.
Lim, F. and A. M. Sun (1980) Science, 210, 908–910.
Nigam, S. C., I. F. Tsao, A. Sakoda and H. Y. Wang (1988) Biotechnol. Techniques, 2, 271–276.
Reuveny, S. A. Mizrahi, M. Kotler and A. Freeman (1983) Biotechnol. Bioeng., 25, 469–480.
Tanaka, H., M. Matsumura and I. A. Veliky (1984) Biotechnol. Bioeng., 26,53–58.
Tyagi, R. D. and T. K. Ghose (1982) Biotechnol., Bioeng., 24, 781–795.
van Wezel, A. L. (1967) Nature, 216, 64–65.
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Cheong, S.H., Park, J.K., Kim, B.S. et al. Microencapsulation of yeast cells in the calcium alginate membrane. Biotechnol Tech 7, 879–884 (1993). https://doi.org/10.1007/BF00156366
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DOI: https://doi.org/10.1007/BF00156366